insulin resistance Type 2 diabetes
Conditions
Interventions
None listed
Sponsors
Academisch Medisch Centrum
Eligibility
Age
18 Years to 99 Years
Inclusion criteria
Inclusion criteria: Type 2 diabetes patients: - Caucasian male or female - A history of T2D as defined by: 1. previous fasting glucose >7.0 mmol/l and fasting glucose >7.0 mmol/l on the day of surgery; OR (previous) HbA1C >48mmol/l; OR 3. use of antidiabetic medication - Scheduled for pancreatic surgery because of pancreatic carcinoma - Subjects should be able to give informed consent;Non-diabetic participants: - fasting plasma glucose
Exclusion criteria
Exclusion criteria: Type 2 diabetes patients: - Use of antibiotics in the past 3 months - Chronic alcohol use - Use of GLP-1 receptor agonists or DPP-4 inhibitors - Inability to understand the study protocol;Non-diabetic participants: - A history of T2D or type 1 diabetes (T1D) - Use of antibiotics in the past 3 months
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Our primary endpoint is the amount of bacterial DNA in pancreatic beta cell tissue. | — |
Secondary
| Measure | Time frame |
|---|---|
| As a secondary endpoint, we will assess fecal and oral microbiota composition and relate this to pancreatic microbiota composition, concentration of bacterial metabolites in pancreatic tissue and markers of inflammation expressed in pancreatic tissue and plasma. Finally, we will study beta cell function ex vivo by culturing beta cells obtained during surgery. | — |
Countries
Netherlands
Outcome results
None listed