Causes and consequences of disrupted bone marrow function following autologous stem cell transplantation, a pilot study

Causes and consequences of disrupted bone marrow function following autologous stem cell transplantation, a pilot study - BM-003/53260

Status

Active, not recruiting

Phases

Unknown

Study type

Observational

Source

NL-OMON

Registry ID

NL-OMON42488

Enrollment

Registered

2015-09-01

Start date

2015-11-05

Completion date

Unknown

Last updated

2024-04-23

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

lymphoma myeloma

Interventions

None listed

Eligibility

Age

18 Years to 99 Years

Inclusion criteria

Inclusion criteria: -Patients diagnosed with lymphoma or myeloma that have been treated with ASCT. -At least 1 year following ASCT -Age >18 years

Exclusion criteria

Exclusion criteria: -Age

Design outcomes

Primary

Measure	Time frame
To obtain information regarding the increased vulnerability of HSPC for chemotherapy, in vitro culture assays will be performed in the absence and presence of cytostatic agents, in the presence and absence of mesenchymal cells (MSC*s). The response of these cells will further be analyzed regarding the induction of stress response (p53) and DNA damage response by performing gene profiling and western blotting. In addition, the genetic and epigenetic background of these cells will be analyzed and correlated with the obtained results by performing targeted sequencing and Chip-seq. For these assays at least 105 cells are required per experiment.	—

Measure

Time frame

To obtain information regarding the increased vulnerability of HSPC for chemotherapy, in vitro culture assays will be performed in the absence and presence of cytostatic agents, in the presence and absence of mesenchymal cells (MSC*s). The response of these cells will further be analyzed regarding the induction of stress response (p53) and DNA damage response by performing gene profiling and western blotting. In addition, the genetic and epigenetic background of these cells will be analyzed and correlated with the obtained results by performing targeted sequencing and Chip-seq. For these assays at least 105 cells are required per experiment.

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Countries

Netherlands

Outcome results