Effect van inspanning met lage glycogeenbeschikbaarheid op de moleculaire respons Not applicable
Conditions
Interventions
Intervention: Two experimental exercise sessions. During both experimental days
subjects will complete an endurance exercise bout in the morning (8.30am)
followed by a resistance exercise session in
Sponsors
Wageningen Universiteit
Eligibility
Age
18 Years to 99 Years
Inclusion criteria
Inclusion criteria: • Male gender between 18 and 30 years of age • BMI between 18.5 - 25 kg/m2 • Participating in regular exercise training (at least 1 exercise session, and a maximum of 3 exercise session per week) • Used to different types of exercise (endurance, resistance and/or concurrent) • Willing to give blood samples • Willing to give muscle biopsies • No use of antibiotics during the study or in the past month • No drug use • Suitable veins for blood withdrawal • Registered by a general-practitioner • Consumption of alcohol beverages is less than 21 per week • No blood donor during the study • No vegetarian and willing to eat meat during the experimental days • Able to be present and participate at both test days and pre testing moments • Not employed, or intern, or working on thesis at the department of Human Nutrition at Wageningen University • Not participating in another scientific study (except EetMeetWeet) • Able to participate during the experimental days en pre tests • Able to speak Dutch
Exclusion criteria
Exclusion criteria: • Medical condition that can interfere with the study outcome (i.e. cardiovascular disease, pulmonary disease, gluten intolerance) • Systolic blood pressure >160 mmHg and/or diastolic blood pressure >100 mmHg • Use of medications known to interfere with gene expression in the muscles (i.e. statins, fenofibrate) • Use of antithrombotic therapy (marcoumar, sintromitis). • Diagnosed diabetes mellitus type 1 or 2 • (Chronic) injuries of the locomotor system that can interfere with the intervention
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| - Phosphorylation of intramuscular proteins - Changes in mRNA expression | — |
Secondary
| Measure | Time frame |
|---|---|
| - A spectrum of post exercise myokines, cytokines and myogenic regulatory factors will be measured. - Muscle glycogen - Glucose, insulin, free fatty acids, cortisol, adrenalin and lactate - Perceived exertion | — |
Countries
Netherlands
Outcome results
None listed