voedingsfysiologie in gezonde personen protein absorption
Conditions
Interventions
Single consumption of 8 different dairy products, on 8 separate test days, in a
portion size that contains 25g protein.
Sponsors
FrieslandCampina Corporate Research
Eligibility
Age
18 Years to 99 Years
Inclusion criteria
Inclusion criteria: • Age over 60y • BMI 21-30 kg/m2 • Non-smoking • Healthy as assessed by the NIZO lifestyle and health questionnaire (*Verklaring leefgewoonten en gezondheid*). • Regular and normal Dutch eating habits as assessed by the NIZO lifestyle and health questionnaire (3 main meals per day) • Veins suitable for cannulation (blood sampling) • Voluntary participation • Having given written informed consent • Willing to comply with study procedures • Accept use of all encoded data, including publication, and the confidential use and storage of all data for 15 years. • Accept disclosure of the financial benefit of participation in the study to the authorities concerned
Exclusion criteria
Exclusion criteria: • Participation in any clinical trial including blood sampling and/or administration of substances up to 30 days before Day 01 of this study • Having a history of medical or surgical events that may significantly affect the study outcome, including: Inflammatory bowel disease, hepatitis, pancreatitis, ulcers, gastrointestinal or rectal bleeding; major gastrointestinal tract surgery such as gastrectomy, gastroenterostomy, or bowel resection; known or suspected gastrointestinal disorders, colon or GI tract cancer • Use of the following medication: glucose lowering drugs, insulin; medication that may impact gastric empyting (e.g. gastric acid inhibitors or laxatives) • Diagnosed with diabetes, being treated for high blood glucose or increased fasting blood glucose (>7 mmol/l in finger prick blood) as assessed during screening visit • Use of protein supplements • Mental status that is incompatible with the proper conduct of the study • A self-reported reported food allergy or sensitivity to dairy ingredients • A self-reported allergy or sensitivity to acetaminophen • Alcohol consumption > 28 units/week and 4/day • Reported unexplained weight loss or weight gain of > 3 kg in the month prior to pre-study screening, or intention to lose weight during the study period • Reported slimming or medically prescribed diet • Recent blood donation (
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Plasma amino acid concentrations before and at 13 timepoints (up till 5 hours) after consumption of the dairy products | — |
Secondary
| Measure | Time frame |
|---|---|
| Plasma glucose and insulin concentrations at 9 out of the 14 time points. Gastric emptying rate, based on 13CO2/12CO2 ratio in expired breath for up to 3 hours after meal consumption, and expressed as half-emptying time and lag phase; and on postprandial acetaminophen concentrations in blood. Subjective rating of appetite sensations (hunger, fullness, satiety, desire to eat and prospective food consumption). | — |
Countries
Netherlands
Outcome results
None listed