Gastrointestinal symptoms
Conditions
Interventions
Subjects will be randomized into one of the two groups. One group will receive
7.5 grams of pectin supplements twice daily for four weeks. A second group will
receive 7.5 grams of placebo supplement
Sponsors
Universiteit Maastricht
Eligibility
Age
18 Years to 99 Years
Inclusion criteria
Inclusion criteria: No gastrointestinal complaints Age between 18 - 40 years and 65 - 75 years BMI between 20 and 30 kg/m2
Exclusion criteria
Exclusion criteria: Use of PPIs, NSAIDs and/or vitamin supplementation, within 14 days prior to testing Administration of probiotic or prebiotic supplements, investigational drugs or participation in any scientific intervention study which may interfere with this study in the 90 days prior to the study Use of antibiotics in the 90 days prior to the study. Pregnancy Smoking
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Intestinal permeability Gut permeability test in which the 24-hour urinary excretion of test substances (i.e. sucrose, lactulose, rhamnose, sucralose and erythritol) will be measured in two separate fractions (0-5 hours and 5-24 hours). Measurement for intestinal permeability will take place at baseline and after the supplementation period of four weeks. | — |
Secondary
| Measure | Time frame |
|---|---|
| Intestinal barrier functioning: • Tight junction structure and proteins in colonic biopies: immunofluorescent labelling and polymerase chain reaction (PCR) quantification of ZO-1, claudin-3 and occludin. Measurements of tight junction structure and proteins in colonic biopsies will take place only after the supplementation period of four weeks. • Ussing chamber experiments in colonic biopsies: transepithelial electrical resistance (TEER), fluorescein- and horseradish peroxidase (HRP) flux. Ussing chamber experiments in colonic biopsies will take place only after the supplementation period of four weeks. • Histology in colonic biopsies by staining mucus/mucins. Histologic assessment of colonic biopsies will take place only after the supplementation period of four weeks. • MicroRNA 29a (MiR-29a) in colonic biopsies. Measurements of MiR-29a will take place only after the supplementation period of four weeks. • Zonulin in blood plasma. Measurements of zonulin will take place at baseline and after the supplementation period of four weeks. Immune system performance • Immune cell infiltration by transcriptomics in colon biopsies. Measurements of immune cell infiltration will take place only after the supplementation period of four weeks. • T-cells and natural killer cells (NK-cells) assessed in PBMC stimulated blood and measured by fluorescence-activated cell sorting (FACS). Measurements of T-cells and NK-cells will take place at baseline and after the supplementation period of four weeks. • Mononuclear cells and platelets in blood plasma. Measurements of mononuclear cells and platelets will take place at baseline and after the supplementation period of four weeks. • Cytokines and chemokines in 24h stimulated whole blood. Measurements of cytokines and chemokines will take place at baseline and after the supplementation period of four weeks. • C-reactive protein (CRP) in blood serum. Measurements of CRP will take place at baseline and after th | — |
Countries
Netherlands
Outcome results
None listed