Blood and Beyond: T-cell immunology in the human lungs

Blood and Beyond: T-cell immunology in the human lungs - Lung T-cells

Status

Recruiting

Phases

Unknown

Study type

Observational

Source

NL-OMON

Registry ID

NL-OMON42231

Enrollment

250

Registered

2015-07-23

Start date

2016-02-02

Completion date

Unknown

Last updated

2024-04-23

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

general principles of respiratory tract disorders including infections and neoplasms lung cancer

Interventions

None listed

Eligibility

Age

18 Years to 99 Years

Inclusion criteria

Inclusion criteria: In order to be eligible to participate in this study, a subject must meet all of the following criteria: - Adult (18+) - Undergoing lobectomy or pneumectomy for an isolated primary lung tumor - Undergoing lung tissue resection for a primary non-infectious pulmonary or pleural disease

Exclusion criteria

Exclusion criteria: - Previous radiotherapy in which the lungs might have been directly in the radiation field - Chemotherapy in the last 6 months - Sleeve lobectomy, wedge resection or metastasectomy

Design outcomes

Primary

Measure	Time frame
Which regulatory circuits modulate T-cell phenotype and function in healthy human lung tissue, lung tumors and peripheral blood	—

Secondary

Measure	Time frame
To answer the primary objective we want to perform the following analyses: 1) Optimization of the isolation of T-cells from tumor and lung tissue (enzymatic digestion, collagenases, mechanical force). 2) Phenotypic comparison of T-cells derived from healthy lung tissue, lung tumors and peripheral blood using multiparameter flow cytometry. 3) Investigation of the localisation of T-cells in healthy lung tissue and lung tumors as well as their accessory cells using confocal microscopy. 4) Investigation of response of lung TRM and T-cells derived from tumor tissue and peripheral blood to activating stimuli (antigen specific/non-specific T-cell receptor stimulation, costimulation and cytokines) to analyse their differential effector functions. 5) Comparison of the transcriptome and clonal composition (both genome wide and T-cell receptor (TCR) sequence) of paired TRM, tumor and peripheral blood derived T-cell populations. 6) Comparison of the proteome of paired TRM, tumor and peripheral blood derived T-cell populations. 7) Combined analyses of the proteomes with the transcriptomes to reveal the proteins that are regulated in a post-transcriptional manner.	—

Measure

Time frame

To answer the primary objective we want to perform the following analyses: 1) Optimization of the isolation of T-cells from tumor and lung tissue (enzymatic digestion, collagenases, mechanical force). 2) Phenotypic comparison of T-cells derived from healthy lung tissue, lung tumors and peripheral blood using multiparameter flow cytometry. 3) Investigation of the localisation of T-cells in healthy lung tissue and lung tumors as well as their accessory cells using confocal microscopy. 4) Investigation of response of lung TRM and T-cells derived from tumor tissue and peripheral blood to activating stimuli (antigen specific/non-specific T-cell receptor stimulation, costimulation and cytokines) to analyse their differential effector functions. 5) Comparison of the transcriptome and clonal composition (both genome wide and T-cell receptor (TCR) sequence) of paired TRM, tumor and peripheral blood derived T-cell populations. 6) Comparison of the proteome of paired TRM, tumor and peripheral blood derived T-cell populations. 7) Combined analyses of the proteomes with the transcriptomes to reveal the proteins that are regulated in a post-transcriptional manner.

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Countries

Netherlands

Outcome results