Bacterial vaginosis and candidiasis - vaginal (yeast) infections
Conditions
Interventions
None listed
Sponsors
Bronovo Ziekenhuis
Eligibility
Age
18 Years to 99 Years
Inclusion criteria
Inclusion criteria: Major criteria: Increase of discharge Odor of discharge;Minor criteria: Color change of discharge Itching, irritation or redness
Exclusion criteria
Exclusion criteria: Postmenopausal women Immune compromised women Women who had a vaginal ultrasound with gel on the day of inclusion Women with complaints of vulva in stead of vagina Women who have been treated with antibiotics or clotrimazole for vaginal infections the last 3 months
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| A. Outcome of culture on Gardnerella vaginalis, C. albicans, yeasts, trichomoniasis and beta-hemolytic streptococcal infections - Positive - Negative - can't be measured B. Outcome gram-stain for bacterial vaginosis - Positive - Negative - can't be measured C. Outcome Multiplex PCR analysis on bacterial vaginosis, atopobium vaginae, lactobacillus spp. Negative 1. Normocenosis: normal vaginal flora Positive Anaerobic disbiosis: bacterial vaginosis with explanation disbalanced vaginal flora, significantly more anaerobe bacteria Doubtful positive 3. Mezocenosis: changed vaginal flora with increased anaerobe bacteria. 4. Unexplained disbiosis: decreased lactobacillus, no increase of anaerobe bacteria. Can't be measured 5. Result of test is unreliable: not specified but will be given to a specific grade of Cq of total bacteria. D. Outcome Multiplex PCR analysis on Candida species: AmpliSens C. albicans, C. glabrata, C. krusei-MULTIPRIME-FRT PCR. - Positive - Negative - can't be measured E. Outcome PCR analysis on Trichomoniasis - Positive - Negative - can't be measured F. Outcome PCR analysis on C. trachomatis - Positive - Negative - can't be measured G. Outcome PCR analysis on N. gonorrhoeae - Positive - Negative - can't be measured | — |
Secondary
| Measure | Time frame |
|---|---|
| Clinical diagnoses based on complaints and vaginal inspection on which we give a treatment. - Bacterial vaginois - Candidiasis - Trichomoniasis - Infection with ß -hemolytic streptococcal group A - C. trachomatis - N. gonorrhoeae Presentation of symptoms - Duration of symptoms - Amount of discharge - Color discharge - Fishy odor of discharge - Fishy odor of discharge after intercourse - Itching - Burning - Vaginal redness of irritation - Pain - Symptoms related to menstruation cycle - Dyspareunia - Seriousness of complaints - Bleeding after intercourse - Symptoms gone after treatment - Dysuria - Abdominal pain Physical examination - Vaginal redness or irritation - Amount of discharge - Color of discharge - Friable white discharge - Frothy white discharge - Purulent discharge - Bleeding cervix at speculum examination - Erythematous cervix - Fishy odor of discharge - Fishy odor after KOH - Acidity of discharge - Clue cells on wet mount - Motile trichomonads on wet mount - Budding yeast, pseudohyphae, and hyphae on a wet mount of the discharge; adding 10 percent potassium hydroxide Time needed to do the following test in minutes - Acidity measurement - whiff-amine test - Microscopial evalution with wet mount or potassium hydroxide Outcome microbioma analysis with bacterial 16S rDNA analysis by nex-gen sequencing (Illumina MiSeq) - Positieve - Negative - Can't be measured | — |
Countries
Netherlands
Outcome results
None listed