insuline resistentie diabetes mellitus type2 obesitas
Conditions
Interventions
The subjects have to consume several standardised meals, including a breakfast
with 13C labeled fatty acids. They have to visit the hospital three times in
fasted state. Several MRS scans and bloods
Sponsors
Medisch Universitair Ziekenhuis Maastricht
Eligibility
Age
18 Years to 99 Years
Inclusion criteria
Inclusion criteria: Lean subjects (study 1): - Age 18-65 years - Normal BMI 18-25 kg/m2 - Stable dietary habits - Generally healthy, no medication use;Obese subjects (study2) - Age 18-65 years, - Obese, BMI 30-35 kg/m2 - Stable dietary habits (no weight-reducing diet last year) - Generally healthy, no medication use
Exclusion criteria
Exclusion criteria: Persons that have any of the following will be excluded from the study: - Any medical condition requiring treatment and/or medication use - Alcohol consumption of more than 20 g per day (± 2 units) or other drug abuse - Unstable body weight (weight gain or loss > 3 kg in the past three months) - Contraindications for MRI scan: • Aneurysm clips • Implanted neural stimulator • Implanted cardiac pacemaker of defibrillator • Cochlear implant • Iron- containing corpora aliena in the eye or brain • Artificial (heart) valves which is contraindicated for MRI • Claustrophobia - Subjects, who do not want to be informed about unexpected medical findings, or do not wish that their treating physician is informed.
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Determination of the lowest dose that allows for sufficient signal to noise ratio and the time of maximal 13C accumulation in liver). Detection and quantification of fractional enrichment (FE) of accumulated 13C-labelled lipids in liver at different time points after dietary intake. | — |
Secondary
| Measure | Time frame |
|---|---|
| • Total- and 13C-enriched blood plasma levels of FFA, triglycerides, glucose. | — |
Countries
Netherlands
Outcome results
None listed