orthopedic differentiation of stem cells
Conditions
Interventions
None listed
Sponsors
Academisch Medisch Centrum
Eligibility
Age
18 Years to 99 Years
Inclusion criteria
Inclusion criteria: Patients - in need of an autologous bone graft harvested of the (anterior or posterior) iliac crest for orthopaedic reason; - understand study proposal and sign informed consent; - > 18 years old
Exclusion criteria
Exclusion criteria: Subjects: - participating in other studies - suffering from an autoimmune disease - suffering from an infection - suffering from a malignancy - receiving or received chemotherapy / other bone marrow suppression medicine
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Avarage number of stem cells available in a bone marrow aspirate and the different cell typesin %. | — |
Secondary
| Measure | Time frame |
|---|---|
| Number of stem cells with bone or vascular generating potentials (through: CD34, CD45, CD90, CD105, CD146, CD271 markers) Colony forming potentials of stem cells (through CFU-F analysis) Genexpressie of the osteoprogenitors (through PCR analyse) Other baseline parameters will be noted to detect potential confounders: sex, smoking, current diets, age and body weight. Study parameters will be compared between patients and also to the existing literature. | — |
Countries
Netherlands
Outcome results
None listed