blood loss hemorraghe
Conditions
Interventions
Phlebotomy (withdrawal of 500 mL volbloed)
Sponsors
Universitair Medisch Centrum Sint Radboud
Eligibility
Age
18 Years to 99 Years
Inclusion criteria
Inclusion criteria: - Male - Age >18 and
Exclusion criteria
Exclusion criteria: - Having donated blood to the blood bank within one year preceding phlebotomy - Significant blood loss from trauma within one year preceding phlebotomy - Having lost > 100 ml of blood due to any cause, within 3 months preceding phlebotomy (not counting blood withdrawn during screening visit) - Having lost > 50 ml of blood due to any cause, within 1 month preceding phlebotomy (not counting blood withdrawn during screening visit) - Having lost >20 ml blood due to any cause, within 1 week preceding phlebotomy (not counting blood withdrawn during screening visit) - Family history of thallasemia, sickle cell disease, hereditary hemochromatosis, or iron refractory iron deficiency anemia - Signs of history of infection within 2 weeks preceding phlebotomy - History of frequent vasovagal response
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Change in ex vivo TNF-* production of whole blood, induced by incubation with LPS, on day -1, 0, 1, 3, 5, 7, 14, 21 and 28 following the withdrawal of 500 ml of blood at the blood bank. | — |
Secondary
| Measure | Time frame |
|---|---|
| - the change in ex vivo production of other cytokines (IL-6, IL10) of whole blood elicited by incubation with LPS, on day -1, 0, 1, 3, 5, 7, 14, 21 and 28 following the withdrawal of 500 ml of blood at the blood bank. - Changes in hemoglobin, hematocrit, white blood cell count and differential on day -1, 0, 1, 3, 5, 7, 14, 21 and 28 following the withdrawal of 500 ml of blood at the blood bank. - Changes in plasma hepcidin concentration in the 28 days following the withdrawal of 500 ml of blood at the blood bank - Changes in other markers of iron homeostasis (serum iron, transferrin saturation, ferritin) in the 28 days following the withdrawal of 500 ml of blood at the blood bank | — |
Countries
Netherlands
Outcome results
None listed