adult-onset diabetes type 2 diabetes mellitus
Conditions
Interventions
None listed
Sponsors
Universiteit Maastricht
Eligibility
Age
18 Years to 99 Years
Inclusion criteria
Inclusion criteria: - Woman with overweight/obesity (body mass index >= 28 kg/m2) and a disturbed glucose metabolism (IFG: 6.1-7.0 mmol/l (or 5.6-7.0 mmol/l and a family history of diabetes) and/or IGT: 2h plasma glucose during OGTT 7.8-11.1 mmol/l) - postmenopausal - Age: 40-65 - Non smoking - A stable bodyweight for at least 3 months (no change in bodyweight:
Exclusion criteria
Exclusion criteria: - diabetes mellitus - cardiovascular diseases - cancer - asthma or bronchitis - liver and/or kidney disease (determined based on ALAT and creatinine levels, respectively) - a disease with a life expectancy 15 units/week) - plans to lose weight (subjects will be asked if they have weight loss plans (e.g. to increase their physical activity level or change diet): a positive answer will lead to exclusion) - participation in sport activities for more than 3 hours/week - Usage of higher doses anti-oxidants (vitamin A, C, E, beta-carteen; standard multi-vitamin supplements are allowed when the concentrations are less than: 800ug/day Vit A, 60mg/day Vit C, 10mg/day Vit E and 400ug/day beta-caroteen) - use of medication with an effect on the glucose metabolism or inflammation - not able to understand the subject information - smoking
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Differences in oxygen tension and further effects for both adipose tissue depots in obese, insulin resistant woman will be investigated. We will perform our experiments in the abdominal and femoral adipose tissue depots and also monitor the blood flow in both depots. Next to this we will also determine the local inflammation. The primary parameter is: - oxygen tension in adipose tissue | — |
Secondary
| Measure | Time frame |
|---|---|
| Secundary parameters are: - adipose tissue blood flow - insulin sensitivity - inflammation markers in adipose tissue (IL-6, TNF-alfa, CD68, leptin, adiponectin, MCP-1, MIF1, PPAR-gamma, CD34 mRNA expressie) - bodyweight, body mass index (BMI) and body composition - whole body adipose tissue distribution (DEXA scan) - adipocyte size - plasma glucose concentration - plasma insulin concentration - glucose tolerance - FFA, triacylglycerol, glycerol concentrations - bloodpressure We will also isolate cells from the biopsies and expose them to different concentrations oxygen. With this experiment we will investigate the effects of oxygen tension on adipocyte metabolism and inflammation. | — |
Countries
Netherlands
Outcome results
None listed