Biomarkers of response Acute changes in blood pressure
Conditions
Interventions
None listed
Sponsors
Universitair Medisch Centrum Utrecht
Eligibility
Age
18 Years to 99 Years
Inclusion criteria
Inclusion criteria: Subject is enrolled in the EPIC-NL cohort or lives close to a busy road Subject enrolled in the EPIC-NL cohort has provided a baseline blood sample by the time of inclusion in the cohort (which is still available) The EXPOsOMICS project obtained approval from EPIC-NL to contact participants via the cohort.
Exclusion criteria
Exclusion criteria: Subject is younger than 50 years or over 70yrs of age by the time of performing the first monitoring session. Subject is a smoker or ex-smoker (less than 6 months) since baseline of cohort inclusion. Subject is currently living with a smoker or ex-smoker (less than 6 months). Subject has a doctor diagnosed chronic disease (e.g. IHD, CVD, COPD, Asthma, Diabetes, Crohn). Subject has/had cancer excluding non-melanoma skin cancer. Subject is restricted in daily activities due to physical limitations. Subject has a job that involves contact with diesel exhaust.
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| PEM measurements: Personal monitoring includes particles smaller than 2.5 µm (PM2.5), the soot content of PM2.5 (marker for diesel emissions) and ultrafine particles (UFP, particulates less than 1 µm). BGI PM2.5 cyclones will be used to collect PM2.5 on a Teflon filter over 24h (van Roosbroeck, 2008). Filters will be weighed to assess ambient PM2.5 concentrations and filter reflectance will be measured to assess soot levels using procedures of the ESCAPE study (Eeftens, 2012). The DiSCMini will be used to measure UFP continuously. At the same time, subjects will carry a belt with a Samsung Galaxy S3 mobile phone with a project developed application to log location and physical activity at 1 second intervals, obtained from the telephone*s internal GPS and Accelerometer. For validation, a regular GPS to track location and an Accelerometer to track physical activity will be used as well. The GPS device will be a Trac King GPS tracker, logging GPS coordinates at 1 second intervals. Actigraph ActiSleep+ Wireless motion sensors will be used for activity measurements, logging activity at 1 second intervals. Biological samples: Blood sample collection 20 ml of whole blood, divided over 3 tubes, will be collected by the standard phlebotomy technique of venipuncture of forearm veins. 10ml will be collected in one EDTA tube, 5 ml will be collected in a Serum tube, and 5 ml will be collected in a PAX-tube. After processing, OMIC analyses will be assessed on the freshly collected samples and on archived blood samples, obtained from the EPIC-NL biobank. Buccal scrapes and Nasal Swabs Since the mouth or the nose are target organs, direct or intermediate responses to air pollution may be detectable in these organs. Therefore, buccal scrape samples will be collected with a special designed sterile plastic scraper and nasal swab samples will be collected from each nostril with a sterile nasal swab. Samples will be analyzed for: - RNA expression - DNA me | — |
Secondary
| Measure | Time frame |
|---|---|
| Home Outdoor measurements: Air monitors will be used to measured air pollution at the subject*s home address. This is allowing a comparison between measured outdoor and personal exposure and a comparison between modelled and measured home outdoor concentrations. The modeled home outdoor concentrations will be calculated based in models, previously developed in the ESCAPE project. Biological samples: Blood pressure measurements Blood pressure will be measured after each PEM session using automatic blood pressure meters (Omron M6, Omron Healthcare Europe BV, Hoofddorp, the Netherlands) according to the recommendations of the American Heart Association (Pickering et al., 2005). The cuff (an adjustable pre-shaped cuff of 22-42 cm, dependent on the mid-upper arm circumference) will be placed at the non-dominant arm. Systolic and diastolic blood pressure will be measured at least two times with 5 minutes intervals according to a standard protocol while the subject is seated. Only if there are two reproducible measurements (difference | — |
Countries
Netherlands
Outcome results
None listed