The effect of cold acclimation on human brown adipose tissue and skeletal muscle mitochondrial uncoupling -a comparison in obesity and type 2 diabetes

The effect of cold acclimation on human brown adipose tissue and skeletal muscle mitochondrial uncoupling -a comparison in obesity and type 2 diabetes - Cold acclimation and brown fat - comparison in obesity and type 2 diabetes

Status

Active, not recruiting

Phases

Unknown

Study type

Interventional

Source

NL-OMON

Registry ID

NL-OMON40307

Enrollment

Registered

2013-11-27

Start date

2014-01-23

Completion date

Unknown

Last updated

2024-04-23

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

type 2 diabetes en overgewicht type 2 diabetes and overweight

Interventions

Cold exposure. For 10 consecutive days the subjects will be exposed to cold (12 C). To easily get used to this, exposure will be 2hours at the 1st day, 4hours at the 2nd day and day 3-10 6hours/day.

Eligibility

Age

18 Years to 99 Years

Inclusion criteria

Inclusion criteria: * Caucasians * Age: o Obese without type 2 diabetes: 18-65 years o Obese with type 2 diabetes: 18-65 years * BMI: 28-35 kg/m2 * Fat percentage: > 23% * Gender: Male * Specifically for obese subjects with type 2 diabetics: o Diagnosed with type 2 diabetes at least 1.5 years before the start of the study o Well-controlled type 2 diabetes: HBA1C

Exclusion criteria

Exclusion criteria: * Participate in physical activity more than 2x/week * Participation in earlier research that included a PET/CT-scan * Radiation therapy due to medical treatment * Unstable body weight (weight gain or loss > 5 kg in the last three months) * Participation in another biomedical study within 1 month before the first screening visit;* Specifically for obese subjects without type 2 diabetes: o Elevated fasting blood glucose level (> 6.1 mmol/L);* Specifically for obese subjects with type 2 diabetes: o Impaired kidney- and/or hepatic function o Diabetes related co-morbidities like cardiovascular diseases, diabetic foot, polyneuropathy, and retinopathy. o Insulin dependent diabetic subjects

Design outcomes

Primary

Measure	Time frame
1. To establish the role of brown adipose tissue in cold-induced thermogenesis before and after a cold acclimation period 2. To study the involvement of skeletal muscle mitochondrial uncoupling in cold-induced thermogenesis 3. To study the effect of chronic cold exposure on the white adipose tissue depot	—

Secondary

Measure	Time frame
1. To study the effect of short-term and chronic cold exposure on relevant blood parameters 2. To establish the effect of short-term and chronic cold exposure on body temperatures and skin temperatures 3. To establish the role of polymorphisms in the UCP-1 and beta-3 receptor gene on BAT activity and cold-induced thermogenesis.	—

Countries

Netherlands

Outcome results

None listed

Source: NL-OMON (via WHO ICTRP)