Adipose tissue in overweight individuals with and without the metabolic syndrome; differences in free fatty acid profile in Fasting and Fed state using 1H-MR-spectroscopy.

Adipose tissue in overweight individuals with and without the metabolic syndrome; differences in free fatty acid profile in Fasting and Fed state using 1H-MR-spectroscopy. - AFFECT 2

Status

Active, not recruiting

Phases

Unknown

Study type

Observational

Source

NL-OMON

Registry ID

NL-OMON39951

Enrollment

Registered

2012-10-30

Start date

2012-12-14

Completion date

Unknown

Last updated

2024-04-29

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

adipose tissue dysfunction overweight

Interventions

None listed

Eligibility

Age

18 Years to 99 Years

Inclusion criteria

Inclusion criteria: 1. Between ages of 18 and 70 years. 2. Male or female 3.a. Metabolic syndrome group: BMI *25 kg/m2 and presence of the metabolic syndrome. 3.b. No metabolic syndrome group: BMI *25 kg/m2 and absence of the metabolic syndrome.

Exclusion criteria

Exclusion criteria: 1. Known cardiovascular disease 2. Known renal, liver or pulmonary disease 3. Diabetes mellitus 4. Use of medication (except for oral anticonceptives, proton pump inhibitor*s, inhalation medication or topical unguents) 5. Pregnancy or lactation 6. Claustrophobia 7. Waist circumference too large to fit MRI (>200cm) 8. Metallic devices in the body of the participant

Design outcomes

Primary

Measure	Time frame
1. Free fatty acid ratios Triglycerides (TG), poly unsaturated fatty acids (PUFA) and total unsaturated fatty acids (TUFA) will be measured with spectroscopy. The PUFA/TG, TUFA/TG and the PUFA/TUFA ratio will be calculated after standardization of the measurements with respect to voxelsize and watercontent. 2. Serum triglycerides vs adipose tissue triglycerides The change in concentration of triglycerides in serum (peripheral blood) will be compared to the change in concentration of triglycerides in the adipose tissue measured with spectroscopy, both before and after an oral fat load.	—

Measure

Time frame

1. Free fatty acid ratios Triglycerides (TG), poly unsaturated fatty acids (PUFA) and total unsaturated fatty acids (TUFA) will be measured with spectroscopy. The PUFA/TG, TUFA/TG and the PUFA/TUFA ratio will be calculated after standardization of the measurements with respect to voxelsize and watercontent. 2. Serum triglycerides vs adipose tissue triglycerides The change in concentration of triglycerides in serum (peripheral blood) will be compared to the change in concentration of triglycerides in the adipose tissue measured with spectroscopy, both before and after an oral fat load.

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Countries

Netherlands

Outcome results