Effect of anticoagulants or procoagulants on thrombin generation in PPP, PRP and whole blood

Effect of anticoagulants or procoagulants on thrombin generation in PPP, PRP and whole blood - Effect of anti- or procoagulants on thrombin generation

Status

Active, not recruiting

Phases

Unknown

Study type

Observational

Source

NL-OMON

Registry ID

NL-OMON39676

Enrollment

175

Registered

2013-03-13

Start date

2013-04-01

Completion date

Unknown

Last updated

2024-04-29

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

bleeding coagulation disorder coagulopathy thrombosis

Interventions

None listed

Eligibility

Age

18 Years to 99 Years

Inclusion criteria

Inclusion criteria: Healthy men and women between 18 and 65 years

Exclusion criteria

Exclusion criteria: - Use of a drug that interferes with coagulation (e.g. oral anticoagulants, oral contraceptives, oral antiplatelet drugs). - A history of bleeding or thrombosis

Design outcomes

Primary

Measure	Time frame
Thrombin generation will be determined in PPP, PRP and whole blood with or without flow. The data that will be collected through these experiments will be analysed with the use of 4 parameters: endogenous thrombin potential (ETP), peak height, time to peak and lag time. The thrombin generation experiments will be performed in the absence and presence of anticoagulant agents or agents that will possibly affect thrombin formation. If necessary, clotting factor determinations will be performed on the stored PPP, these can be antigen determinations or functional determinations. Control experiments need to be carried out in order to check the quality of the blood/plasma by using the standard procedures of the lab: e.g. cell count, flow cytometry, aggregometry, clotting*	—

Measure

Time frame

Thrombin generation will be determined in PPP, PRP and whole blood with or without flow. The data that will be collected through these experiments will be analysed with the use of 4 parameters: endogenous thrombin potential (ETP), peak height, time to peak and lag time. The thrombin generation experiments will be performed in the absence and presence of anticoagulant agents or agents that will possibly affect thrombin formation. If necessary, clotting factor determinations will be performed on the stored PPP, these can be antigen determinations or functional determinations. Control experiments need to be carried out in order to check the quality of the blood/plasma by using the standard procedures of the lab: e.g. cell count, flow cytometry, aggregometry, clotting*

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Countries

Netherlands

Outcome results