bacteraemia bloodstream infection sepsis (in case blood stream infection sepsis is regularly diagnosed)
Conditions
Interventions
None listed
Sponsors
Vrije Universiteit Medisch Centrum
Eligibility
Age
18 Years to 99 Years
Inclusion criteria
Inclusion criteria: - Age >= 18 years - Bacteraemia with at least one blood culture with growth of Gram-negative bacteria or Gram-positive cocci in chain observed with microscopy. - Bacteraemia with at least one blood culture with growth of Gram-positive cocci (in clusters) and at least one of the following syndromes suggestive of S. aureus infection: arthritis, wound infection, thrombophlebitis, meningitis, soft tissue infection, vertebral osteomyelitis, splenic abscess or other microbiological evidence for S. aureus infection
Exclusion criteria
Exclusion criteria: - Blood culture obtained >48 hours - Intravenous antibiotic therapy >48 hours - Haematological disorder - Anaemia (an Hb below 5.5 mmol/L) - Participation in BLENT-study of patients with endocarditis - Previous participation in this study
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| The diagnostic performance of each molecular assay is determined by the sensitivity, specificity, positive predictive value, negative predictive value as compared to culture as a golden standard. Inter-assay variation is determined by measure of agreement and calculation of kappa value. | — |
Secondary
| Measure | Time frame |
|---|---|
| To assess the putative applicability of the molecular assays in routine molecular diagnostics with regard to costs, hands on time and difficulty of the protocol in routine use. | — |
Countries
Netherlands
Outcome results
None listed