The role of surgery- induced inflammatory mediators in macrophage activation

The role of surgery- induced inflammatory mediators in macrophage activation - Surgery-induced macrophage activation

Status

Active, not recruiting

Phases

Unknown

Study type

Observational

Source

NL-OMON

Registry ID

NL-OMON39271

Enrollment

Registered

2012-04-17

Start date

2013-05-23

Completion date

Unknown

Last updated

2024-04-29

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

colon cancer Colorectal carcinoma

Interventions

None listed

Eligibility

Age

18 Years to 99 Years

Inclusion criteria

Inclusion criteria: Resection of primary colorectal carcinoma.

Exclusion criteria

Exclusion criteria: Excisting infection: Crohn's disease or ulcerative colitis

Design outcomes

Primary

Measure	Time frame
- Capacity of pre- per- and post operative plasma to activate macrophages in vitro. Endpoints are production of reactive oxygen species (ROS) and conversion of MTT to formazan (this allows to assess the viability of cells). - Identification of the inflammatory factors released after surgery that are responsible for macrophage activation. Endpoints are decrease of ROS production and conversion of MTT in the presence of blocking agents against inflammatory mediators.	—

Measure

Time frame

- Capacity of pre- per- and post operative plasma to activate macrophages in vitro. Endpoints are production of reactive oxygen species (ROS) and conversion of MTT to formazan (this allows to assess the viability of cells). - Identification of the inflammatory factors released after surgery that are responsible for macrophage activation. Endpoints are decrease of ROS production and conversion of MTT in the presence of blocking agents against inflammatory mediators.

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Countries

Netherlands

Outcome results