metagenomics, resistomics (feces, nasopharyngeal) bacterial colonisation in children with cystic fibrosis
Conditions
Interventions
None listed
Sponsors
Universitair Medisch Centrum Utrecht
Eligibility
Age
2 Years to 11 Years
Inclusion criteria
Inclusion criteria: 1. diagnosis of cystic fibrosis, proven by positive sweat chloride test and DNA-analysis 2. age
Exclusion criteria
Exclusion criteria: 1. Other underlying disease or prematurity (
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| We expect in-depth knowledge about nasopharyngeal colonization dynamics and species interaction in young healthy children and children with CF, as well as the sequential relationship with clinical respiratory symptoms in infants with CF. Besides we aim to unravel the effect of antibiotic treatment on divergence of the natural protective microflora in relation to disease progression as well as it*s effect on the evolution of the resistome and resistance in respiratory pathogens in this young population. Lastly we want to determine the mucosal immune response (antibody development) against common respiratory pathogens in children (e.g. pneumococcus) in relation to colonization and vaccination in this patient group. | — |
Secondary
| Measure | Time frame |
|---|---|
| We will collect saliva samples to investigate the mucosal immune response in relation to natural boosting by pneumococcal colonization and childhood vaccinations in children with CF compared to controls (saliva IgA and IgG). In the present drSNUIT study we observe events of nasopharyngeal recolonization with the same pathogen, therefore we intend to investigate the correlation between (re)colonization and species-specific local antibody responses in saliva (local IgA-production). It is interesting to know if nasopharyngeal colonization elecits protective antibody levels against recolonization | — |
Countries
Netherlands
Outcome results
None listed