The effect of fasting-induced insulin resistance on brown adipose tissue activity, non-shivering thermogenesis and skeletal muscle mitochondrial uncoupling.

The effect of fasting-induced insulin resistance on brown adipose tissue activity, non-shivering thermogenesis and skeletal muscle mitochondrial uncoupling. - Effect of insulin resistance on brown adipose tissue activity.

Status

Active, not recruiting

Phases

Unknown

Study type

Interventional

Source

NL-OMON

Registry ID

NL-OMON39127

Enrollment

Registered

2012-05-07

Start date

2012-05-01

Completion date

Unknown

Last updated

2024-04-29

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

Geen na

Interventions

Fasting period of 54 hours to induce severe insulin resistance in healthy subjects. The insulin resistance is temporarily and fully reversible.

Eligibility

Age

18 Years to 99 Years

Inclusion criteria

Inclusion criteria: Healthy, lean (BMI 18-25 kg/m2) adults Age: 18-30 years Male and female For females: use of a specific oral contraceptive (Microgynon 30 or levonorgestrel/ethinylestradiol) Caucasian Sedentary (=

Exclusion criteria

Exclusion criteria: Cardiovascular diseases Hypertension (systolic/diastolic blood pressure > 140/90) Hypotension (systolic/diastolic blood pressure 5.6 mmol/l) Hyperthyroidism Pregnancy Participation in earlier research or medical examinations that included PET/CT scanning Radiation therapy due to medical treatment Unstable body weight (weight gain or loss >3kg in the past 2 months) Any known or suspected obstructive disease of the gastrointestinal tract, hypo motility disorders of the gastrointestinal tract or previous gastrointestinal surgery.

Design outcomes

Primary

Measure	Time frame
The main study parameters are: - Standard uptake value (SUV) of BAT: This is used as a measure of BAT glucose uptake and BAT activity and will be measured by means of FDG-PET/CT scanning. - Skeletal muscle mitochondrial respiration/uncoupling: This will be determined in skeletal muscle samples using the Oroboros 2k Oxygraph instrument present in our laboratroy. - Energy expenditure: This will be measured by means of indirect calorimetry using a ventilated hood. - Ex vivo skeletal muscle glucose oxidation: This will be measured to determine insulin sensitivity. A glucose oxidation assay with insulin stimulation will be performed in skeletal muscle biopsies.	—

Measure

Time frame

The main study parameters are: - Standard uptake value (SUV) of BAT: This is used as a measure of BAT glucose uptake and BAT activity and will be measured by means of FDG-PET/CT scanning. - Skeletal muscle mitochondrial respiration/uncoupling: This will be determined in skeletal muscle samples using the Oroboros 2k Oxygraph instrument present in our laboratroy. - Energy expenditure: This will be measured by means of indirect calorimetry using a ventilated hood. - Ex vivo skeletal muscle glucose oxidation: This will be measured to determine insulin sensitivity. A glucose oxidation assay with insulin stimulation will be performed in skeletal muscle biopsies.

—

Secondary

Measure	Time frame
Secondary study parameters include: -Body temperatures: Skin temperatures will be measured by means of iButtons and core temperature will be determined by ingestion of a telemetric pill. - Skin perfusion: This will be measured by means of LaserDoppler flowmetry. - Blood parameters: Venous blood will be drawn by means of a catheter placed in the antercubital vein of the underarm. By using radioimmunoassay and high performance liquid chromatography blood parameters will be analyzed. - UCP-1 and beta3-receptor polymorphims	—

Measure

Time frame

Secondary study parameters include: -Body temperatures: Skin temperatures will be measured by means of iButtons and core temperature will be determined by ingestion of a telemetric pill. - Skin perfusion: This will be measured by means of LaserDoppler flowmetry. - Blood parameters: Venous blood will be drawn by means of a catheter placed in the antercubital vein of the underarm. By using radioimmunoassay and high performance liquid chromatography blood parameters will be analyzed. - UCP-1 and beta3-receptor polymorphims

—

Countries

Netherlands

Outcome results

None listed

Source: NL-OMON (via WHO ICTRP)