Ischemia/reperfusion injury. Graft failure after transplantation.
Conditions
Interventions
None listed
Sponsors
Leids Universitair Medisch Centrum
Eligibility
Age
18 Years to 99 Years
Inclusion criteria
Inclusion criteria: Recipients of a kidney graft by kidney tranplantation. (20 recipients) Living donors (10 donors for a pre-surgical measurement of bloodflow in the a.renalis, non-invasive by echo-doppler).4 of these living donors for 1 arterio and 1 venous sample as control measurement during surgery.
Exclusion criteria
Exclusion criteria: Increased risk of bleeding.
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Metabolic activity of the kidney as measured by pO2, pCO2, lactate, glucose and full lipid profile. The respiratory quotients (RQ) for every time-point will be calculated. | — |
Secondary
| Measure | Time frame |
|---|---|
| Kidney biopsies taken before (routine clinical biopsy) and after reperfusion (this study) will be collected in all patients. Biopsy specimens will be snap frozen and processed to make it suitable for NMR. By NMR different intermediates of the citric acid cycle will be quantified, to be able to estimate activity of aerobic metabolism and more accurately to define where potential problems are. Since measurements are dependent on flow kinetics and perfusion of the kidney, flow in the renal artery and microvascular perfusion will be measured as well. Both will be compared between living and cadaveric donor kidneys. Measurements of metabolism will be related to microvascular perfusion We will also measure renal blood flow in the living donor and compare this to the renal blood flow through the same kidney after transplantation. | — |
Countries
Netherlands
Outcome results
None listed