Functional immunity against different malaria life stages in expatriates naturally exposed to P. falciparum.

Functional immunity against different malaria life stages in expatriates naturally exposed to P. falciparum. - IMEX

Status

Active, not recruiting

Phases

Unknown

Study type

Observational

Source

NL-OMON

Registry ID

NL-OMON38663

Enrollment

Registered

2013-11-14

Start date

2013-10-01

Completion date

Unknown

Last updated

2024-04-29

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

malaria immunity

Interventions

None listed

Eligibility

Age

18 Years to 99 Years

Inclusion criteria

Inclusion criteria: Fifty healthy adults who have lived in malaria endemic regions for a minimum of 5 years.

Exclusion criteria

Exclusion criteria: Lived in malaria endemic region for less than 5 years

Design outcomes

Primary

Measure	Time frame
A single venous blood sample (32mL) will be taken along with a short questionnaire with questions about malaria exposure and age at first malaria exposure. After anonimisation, peripheral blood mononuclear cell (PBMC these samples will be used to: i) assess functional transmission blocking immunity by standard membrane feeding assay; ii) determine (a reduction in) hepatocyte invasion by sporozoites in the presence of isolated immunoglobulins (IgG), iii) determine the production of interferon-gamma (IFN-), tumor necrosis factor (TNF-*) and interleukin-2 (IL-2) in PBMC subpopulations after stimulation with plasmodium infected red blood cells, sporozoites and malaria antigens; iv) determine the antibody profile against a panel of sexual stage, bloodstage and liverstage antigens by protein microarray.	—

Measure

Time frame

A single venous blood sample (32mL) will be taken along with a short questionnaire with questions about malaria exposure and age at first malaria exposure. After anonimisation, peripheral blood mononuclear cell (PBMC these samples will be used to: i) assess functional transmission blocking immunity by standard membrane feeding assay; ii) determine (a reduction in) hepatocyte invasion by sporozoites in the presence of isolated immunoglobulins (IgG), iii) determine the production of interferon-gamma (IFN-*), tumor necrosis factor * (TNF-*) and interleukin-2 (IL-2) in PBMC subpopulations after stimulation with plasmodium infected red blood cells, sporozoites and malaria antigens; iv) determine the antibody profile against a panel of sexual stage, bloodstage and liverstage antigens by protein microarray.

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Secondary

Measure	Time frame
-	—

Countries

Netherlands

Outcome results