Vespid venom allergy wasp venom allergy
Conditions
Interventions
Blood sampling for gene expression analysis before and after the sting
challenge test.
Sponsors
Universitair Medisch Centrum Groningen
Eligibility
Age
18 Years to 99 Years
Inclusion criteria
Inclusion criteria: * A clear positive history of a systemic response grade II, III or IVa after a vespid sting * Vespid specific serum IgE > 0.35 kUA/l or a positive intracutaneous skin test for vespid venom (at =0.7) written informed consent
Exclusion criteria
Exclusion criteria: * A positive history of a systemic response grade IVb according to Mueller (13) after a vespid sting * Age under 18 years or above 65 years * Incapacitated subjects * Severe cardiopulmonary disease (clinical evidence of congestive heart failure, coronary disease or severe hypertension) * Insufficiently controlled asthma based on history, asthma control questionnaire and if the observed FEV1 is 70% of predicted. * Conditions that influence the immune system (immune deficiencies, malignancy, auto-immune diseases, flulike dissease) * Severe kidney failure * Interfering medication with the outcome or recovery from a systemic reaction (β-blockers and immunosuppressive drugs) * Pregnancy(measured before inclusion by β-hCG) * Mastocytosis
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Main study parameter: Genes for which the expression is significantly different between reactors and non-reactors based on a log2 fold change > 2 in gene expression, identify the corresponding functional annotation and building a corresponding naïve Bayes prediction model to identify patients at risk for a re-systemic reaction. | — |
Secondary
| Measure | Time frame |
|---|---|
| Secondary study parameter: Genes for which the expression after the sting challenge is significantly different compared to base line expression levels and identify the corresponding functional annotation. | — |
Countries
Netherlands
Outcome results
None listed