sepsis infection in the blood sepsis
Conditions
Interventions
In the hypoxia group: the subjects will breathe an individualized mix of
nitrogen and room air for three-and-a-half hours using an air-tight respiratory
helmet. The gas mixture will be adjusted to a
Sponsors
Universitair Medisch Centrum Sint Radboud
Eligibility
Age
18 Years to 99 Years
Inclusion criteria
Inclusion criteria: -Age >=18 and
Exclusion criteria
Exclusion criteria: -Use of any medication, smoking -History, signs or symptoms of cardiovascular disease, history of atrial or ventricular arrhythmia, (Family) history of myocardial infarction or stroke under the age of 65 years, cardiac conduction abnormalities on the ECG consisting of a 2nd degree atrioventricular block or a complex bundle branch block, hypertension (defined as RR systolic > 160 or RR diastolic > 90), hypotension (defined as RR systolic 120 µmol/l), Liver enzyme abnormalities alkaline phosphatase>230 U/L and/or ALT>90 U/L -Medical history of any obvious disease associated with immune deficiency, pre-existent lung disease or asthma -CRP > 20 mg/L, WBC > 12x109/L, or clinically significant acute illness, including infections, within 4 weeks before endotoxemia day -Participation in a drug trial or donation of blood 3 months prior to the experiment, use of recreational drugs within 21 days prior to experiment day, visit to altitude >1500m within 4 weeks prior to the experiment, air travel with flight time over 3 hours within 4 weeks prior to the experiment, history of acute mountain sickness, recent hospital admission or surgery with general anaesthesia (
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| The main study endpoint is the difference in HIF1a mRNA in circulating leukocytes between hypoxia and hyperoxia. | — |
Secondary
| Measure | Time frame |
|---|---|
| Secondary objectives are HIF1a protein and aHIF mRNA expression in circulating leukocytes, measures of ROS, and plasma levels of inflammatory cytokines. | — |
Countries
Netherlands
Outcome results
None listed