Observational study to investigate the regional differences in abdominal adipose tissue depots regarding function and morphology, in relation to systemic inflammation, endothelial dysfunction and atherosclerosis in patients undergoing elective abdominal aortic surgery.;korte naam: Adipose tissue Dysfunction in Patients in relation to ObeSity and vascular Events - study

Observational study to investigate the regional differences in abdominal adipose tissue depots regarding function and morphology, in relation to systemic inflammation, endothelial dysfunction and atherosclerosis in patients undergoing elective abdominal aortic surgery.;korte naam: Adipose tissue Dysfunction in Patients in relation to ObeSity and vascular Events - study - The ADIPOSE-II study

Status

Active, not recruiting

Phases

Unknown

Study type

Observational

Source

NL-OMON

Registry ID

NL-OMON38295

Enrollment

Registered

2010-09-02

Start date

2010-10-25

Completion date

Unknown

Last updated

2024-05-06

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

abdominal aortic aneurysm or localized widening (dilation) of a blood vessel

Interventions

None listed

Eligibility

Age

18 Years to 99 Years

Inclusion criteria

Inclusion criteria: 1.Written and signed informed consent. 2.Male or female (postmenopausal) patients 3.Indicated for elective abdominal aortic surgery in the University Medical Center Utrecht

Exclusion criteria

Exclusion criteria: 1.Thyroid disease (TSH > 5 mU/L with clinical symptoms of hypothyroidism) 2.Hepatic disease (ASAT or ALAT > 2 times the upper limit of normal) 3.History of malignancy (in the last 2 years) 4.Use of thiazolidinediones (TZD) 5.Use of immune suppressive medication (equivalent of prednisolon >= 10 mg /day) 6.History of liposuction

Design outcomes

Primary

Measure	Time frame
Differences in function and morphology between the distinct abdominal adipose tissue depots, by comparing: - The secretion profile of adipocytokines ex vivo measured with LUMINEX and ELISA - The mean difference in basal mRNA expression of adipocytokines of the adipose tissues, measured by quantitative RT-PCR and - The mean differences in morphology, measured by number and size of adipocytes, capillaries, inflammatory cells and ischemia. - The mean differences in number of specific inflammatory cells present in subcutaneous versus visceral adipose tissue.	—

Measure

Time frame

Differences in function and morphology between the distinct abdominal adipose tissue depots, by comparing: - The secretion profile of adipocytokines ex vivo measured with LUMINEX and ELISA - The mean difference in basal mRNA expression of adipocytokines of the adipose tissues, measured by quantitative RT-PCR and - The mean differences in morphology, measured by number and size of adipocytes, capillaries, inflammatory cells and ischemia. - The mean differences in number of specific inflammatory cells present in subcutaneous versus visceral adipose tissue.

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Secondary

Measure	Time frame
1. Mean differences in quantity of the distinct adipose tissue depots measured from images of routinely performed CT scans, correlated to differences in functional and/or morphological parameters of the adipose tissue depots like adipocytokine secretion pattern or mean adipocyte cell size. 2. Difference between independent correlations of the different AT depots with clinical parameters of the metabolic syndrome. Therefore functional and/or morphological parameters of each adipose tissue depot (like adipocytokine secretion pattern or mean adipocyte cell size) will be correlated to clinical parameters of the patient and correlations between the distinct adipose tissue depots will be compared. 3. Mean differences in function and morphology of the distinct adipose tissue depots, correlated to systemic plasma concentrations of bioactive mediators.	—

Measure

Time frame

1. Mean differences in quantity of the distinct adipose tissue depots measured from images of routinely performed CT scans, correlated to differences in functional and/or morphological parameters of the adipose tissue depots like adipocytokine secretion pattern or mean adipocyte cell size. 2. Difference between independent correlations of the different AT depots with clinical parameters of the metabolic syndrome. Therefore functional and/or morphological parameters of each adipose tissue depot (like adipocytokine secretion pattern or mean adipocyte cell size) will be correlated to clinical parameters of the patient and correlations between the distinct adipose tissue depots will be compared. 3. Mean differences in function and morphology of the distinct adipose tissue depots, correlated to systemic plasma concentrations of bioactive mediators.

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Countries

Netherlands

Outcome results