Complement activation after gut ischemia-reperfusion injury (GI I/R) in man

Complement activation after gut ischemia-reperfusion injury (GI I/R) in man - Complement activation after GI I/R

Status

Recruiting

Phases

Unknown

Study type

Observational

Source

NL-OMON

Registry ID

NL-OMON38154

Enrollment

139

Registered

2006-06-26

Start date

2006-11-01

Completion date

Unknown

Last updated

2024-06-18

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

Gut ischemia/reperfusion

Interventions

None listed

Eligibility

Age

18 Years to 99 Years

Inclusion criteria

Inclusion criteria: Adult patients Major upper abdominal surgery Major colorectal surgery right hemicolectomy

Exclusion criteria

Exclusion criteria: We will not include patients known with Inflammatory Bowel Disease (IBD), because of pre-existing effects of the disease on the inflammatory status of the gut. Acute major abdominal procedures will not be included, because of the possible pre-existing effects of trauma or hemorrhage on the gut. We will exclude those patients who have not given informed consent.

Design outcomes

Primary

Measure	Time frame
Firstly, we will compare cellular damage (I-FABP, ILBP, ZO-1) in the tissue, which was subjected to ischemia-reperfusion with the tissue, which was not subjected to ischemia-reperfusion. Secondly, we will compare complement activation (eg MBL, TCC) in the tissue, which was subjected to ischemia-reperfusion with the tissue, which was not subjected to ischemia-reperfusion. Thirdly, we will compare inflammation (eg Calprotectin, MPO, NGAL) in the tissue, which was subjected to ischemia-reperfusion with the tissue, which was not subjected to ischemia-reperfusion. Fourth, we will study the consequences of intestinal I/R on intestinal barrier function loss, by comparing the translocation of sugars from the gut lumen to the circulation, before ischemia, after ischemia and after 30 and 120 minutes reperfusion. Fifth, we will study macroscopic mucosal changes during intestinal IR using a endo-videocapsule. Data will be analyzed in collaboration with the department of Gastroenterology. At last we want to study the gene expression patterns in tissue samples of IR exposed intestine, in order to identify possible targets for preventive and therapeutic interventions.	—

Measure

Time frame

Firstly, we will compare cellular damage (I-FABP, ILBP, ZO-1) in the tissue, which was subjected to ischemia-reperfusion with the tissue, which was not subjected to ischemia-reperfusion. Secondly, we will compare complement activation (eg MBL, TCC) in the tissue, which was subjected to ischemia-reperfusion with the tissue, which was not subjected to ischemia-reperfusion. Thirdly, we will compare inflammation (eg Calprotectin, MPO, NGAL) in the tissue, which was subjected to ischemia-reperfusion with the tissue, which was not subjected to ischemia-reperfusion. Fourth, we will study the consequences of intestinal I/R on intestinal barrier function loss, by comparing the translocation of sugars from the gut lumen to the circulation, before ischemia, after ischemia and after 30 and 120 minutes reperfusion. Fifth, we will study macroscopic mucosal changes during intestinal IR using a endo-videocapsule. Data will be analyzed in collaboration with the department of Gastroenterology. At last we want to study the gene expression patterns in tissue samples of IR exposed intestine, in order to identify possible targets for preventive and therapeutic interventions.

—

Secondary

Measure	Time frame
Not applicable.	—

Countries

Netherlands

Outcome results