Leukocyte dynamics in ageing: in vivo labelling of dividing leukocytes using deuterated water

Leukocyte dynamics in ageing: in vivo labelling of dividing leukocytes using deuterated water - Leukocyte dynamics in ageing

Status

Active, not recruiting

Phases

Unknown

Study type

Observational

Source

NL-OMON

Registry ID

NL-OMON38081

Enrollment

Registered

2011-03-29

Start date

2011-08-12

Completion date

Unknown

Last updated

2024-05-06

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

immunologische veroudering lymphopenia

Interventions

None listed

Eligibility

Age

18 Years to 99 Years

Inclusion criteria

Inclusion criteria: 5 healthy young individuals; 20-25 years of age 10 healthy older individuals; at least 60 years of age

Exclusion criteria

Exclusion criteria: (i) Infection with human immunodeficiency virus (HIV), hepatitis B virus (HBV), or hepatitis C virus (HCV); (ii) Other severe infections, e.g. malaria, infectious mononucleosis, a sexually transmitted disease (STD); (iii) Hayfever or allergy; (iv) Asthma or COPD; (v) Diabetes; (vi) Auto-immune disease, e.g. rheumatoid arthritis; (vii) Heart problems, or a history of heart problems; (viii) Kidney problemns, or a history of kidney problems; (ix) Chest pain or shortage of breath, in rest or during exercise; (x) Body weight of less than 50 kg; (xi) Use of medication, with the exception of (a) analgesics when taken less frequent than weekly (paracetamol, ibuprofen, aspirin) (b) contraceptives; (xii) History of cancer; (xiii) Pregnancy or parent's wish in the coming year; (xiv) Excessive alcohol consumption; (xv) Drug use; (xvi) Changing and unsafe sexual contacts; (xvii) Sexual contact with an individual infected with HIV or another STD within the last 12 months; (xviii) Extreme sensitivity to car-sickness and/or sea-sickness

Design outcomes

Primary

Measure	Time frame
The main parameter of the study is the amount of deuterium (label) that the different leukocyte populations have incorporated in their DNA by cell division at a given time. For this purpose blood withdrawals are done both in the period during which participants drink 2H2O (uplabelling phase) and in the period after stopping with 2H2O intake (downlabelling phase). Data obtained during uplabelling and downlabelling phases can be interpreted by mathematical models that describe the dynamics of leukocyte populations.	—

Measure

Time frame

The main parameter of the study is the amount of deuterium (label) that the different leukocyte populations have incorporated in their DNA by cell division at a given time. For this purpose blood withdrawals are done both in the period during which participants drink 2H2O (uplabelling phase) and in the period after stopping with 2H2O intake (downlabelling phase). Data obtained during uplabelling and downlabelling phases can be interpreted by mathematical models that describe the dynamics of leukocyte populations.

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Secondary

Measure	Time frame
With the obtained materials several in vitro immunological test will be performed, including determination of blood- and plasma levels and analysis of cell phenotypes and cell numbers within subpopulations.	—

Countries

Netherlands

Outcome results