Hepatic metabolism Hepatic metabolism The metabolic process of the liver
Conditions
Sponsors
Universiteit Maastricht
Eligibility
Age
18 Years to 99 Years
Inclusion criteria
Inclusion criteria: Male sex - Age 40-65 years - Overweight/obese, BMI 25-35 kg/m2 - Healthy - Stable dietary habits - Must be on sulphonylurea or metformin therapy for at least 6 months with constant dose for at least 2 months, or on a dietary treatment for at least 6 months - Well-controlled diabetes: fasting plasma glucose concentration >= 7.0 mmol/l and
Exclusion criteria
Exclusion criteria: Persons that have any of the following will be excluded from the study: - Female sex - Haemoglobin 2 hours total per week - Liver disease, other than NAFLD - Hypertension: blood pressure > 160 mmHg systolic or 100 mmHg diastolic - Cardiac problems, such as angina pectoris, cardiac infarction and arrhythmias - First degree relatives with history of liver disease and diabetes mellitus - Alcohol consumption of more than 20 g per day (± 2 units) - Unstable body weight (weight gain or loss > 3 kg in the past three months) - Participation in another biomedical study within 1 month prior to the screening visit - Insulin use - Contraindications for MRI scan: • Central nervous system aneurysm clips • Implanted neural stimulator • Implanted cardiac pacemaker of defibrillator • Cochlear implant • Iron- containing corpora aliena in the eye or brain • Hearing aids and artificial (heart) valves which is contraindicated for MRS - Subjects, who do not want to be informed about unexpected medical findings, or do not wish that their treating physician is informed, cannot participate in the study - Liver fat content lower than 5.56%
Design outcomes
Secondary
| Measure | Time frame |
|---|---|
| - Substrate oxidation - Blood plasma levels of FFA, triglycerides, glucose and catecholamine - maximal aerobic capacity (VO2max) - body composition (hydrostatic weighing) | — |
Primary
| Measure | Time frame |
|---|---|
| - hepatic lipid content (determined non-invasively by 1H-MRS, ratio of the signal intensity of the CH2-lipid peak to the signal intensity of the water peak) before and after the high FFA condition compared to the low FFA condition and hepatic ATP-and Pi concentrations associated with it. - hepatic lipid content before exercise compared to after exercise | — |
Countries
Netherlands
Outcome results
None listed