Insulin-resistance microvascular dysfunction
Conditions
Interventions
None listed
Sponsors
Vrije Universiteit Medisch Centrum
Eligibility
Age
18 Years to 99 Years
Inclusion criteria
Inclusion criteria: -Age 18-55 years -BMI >30 AND a waist-circumference of >94 cm in males, >80 cm in females, or BMI
Exclusion criteria
Exclusion criteria: -Diabetes or impaired glucose tolerance (FPG > 6.1 mmol/l) -Hypertension or other cardiovascular disease (stroke, coronary artery disease, peripheral vascular disease, heart failure) in medical history -Use of medication for hypertension or diabetes, as well as use of medication known to disturb endothelial function or glucose metabolism -Physical exercise (sporting) more than two times a week -Pregnancy or intention to become pregnant during the study period -Smoking
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| The first main study parameter is the rate of muscle microvascular recruitment on insulin infusion, as measured by change of signal strength during contrast enhanced ultrasonography. The second main outcome is the amount of mPAT in muscle biopsies obtained from lean and obese subjects, quantified by confocal microscopy. The third main outcome is the microvascular vasoreactivity of human muscle arterioles as measured in pressure myography ex vivo. The fourth main outcome is the effects of mPAT on the insulin signalling cascade, as measured by Western blotting of mPAT and comparing results of Western blots from subjects with a normal vasoreactivity and those of subjects with perturbed vasoreactivity. | — |
Countries
Netherlands
Outcome results
None listed