Cutaneous oxygenation and mitochondrial respirometry measurements, using the Protoporphyrin IX - Triplet State Lifetime Technique

Cutaneous oxygenation and mitochondrial respirometry measurements, using the Protoporphyrin IX - Triplet State Lifetime Technique - Mitochondrial oxygen measurements in the skin

Status

Recruiting

Phases

Unknown

Study type

Observational

Source

NL-OMON

Registry ID

NL-OMON37834

Enrollment

Registered

2012-07-05

Start date

2012-12-13

Completion date

Unknown

Last updated

2024-04-29

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

gezonde vrijwilligers Mitochondrial oxygen measurements in the skin standard values

Interventions

None listed

Eligibility

Age

18 Years to 99 Years

Inclusion criteria

Inclusion criteria: - Speaking Dutch - Age between 18 and 30 years. - Healthy subjects

Exclusion criteria

Exclusion criteria: - Non healthy subjects

Design outcomes

Secondary

Measure	Time frame
The practical applicability of the method will be tested by the researcher. If necessary adjustments will be made. The raw data of the measurements will be stored for extended off-line analysis of the signal quality. If necessary, we will look at opportunities to optimize the equipment. The structure of the fluorescent signal, determines by means of the intensity of the detected signal (V) divided by time (minutes), expressed in V / minute. The influence of skin temperature to the search parameters (mitoPO2, and mitoVO2 mitoP50), will be determined at two different skin temperatures (34 and 40 degrees celsius ).	—

Measure

Time frame

The practical applicability of the method will be tested by the researcher. If necessary adjustments will be made. The raw data of the measurements will be stored for extended off-line analysis of the signal quality. If necessary, we will look at opportunities to optimize the equipment. The structure of the fluorescent signal, determines by means of the intensity of the detected signal (V) divided by time (minutes), expressed in V / minute. The influence of skin temperature to the search parameters (mitoPO2, and mitoVO2 mitoP50), will be determined at two different skin temperatures (34 and 40 degrees celsius ).

—

Primary

Measure	Time frame
The mean and standard deviation of mitoPO2 will be calculated in mmHg. The mitoVO2 and mitoP50 will be determined by a decrease in mitoPO2 after oxygen supply is blocked to the cells . The mitoPO2 will be recorded before during, and after a 90 second compression. The mitoVO2 is calculated from the decay of the mitoPO2 slope. With the Michaelis-Menten equation we can calculate MitoP50 in mmHg.	—

Countries

Netherlands

Outcome results