age-related muscle loss sarcopenia
Conditions
Interventions
protein type, bovine milk or minced beef
Sponsors
Medisch Universitair Ziekenhuis Maastricht
Eligibility
Age
18 Years to 99 Years
Inclusion criteria
Inclusion criteria: Males Aged between 18-35 years Healthy, recreationally active BMI
Exclusion criteria
Exclusion criteria: Smoking Allergies to milk proteins (whey or casein) Vegetarians Female Arthritic conditions A history of neuromuscular problems Previous participation in amino acid tracer studies
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| The main study endpoint is the muscle protein synthesis rate, expressed as fractional synthetic rate (FSR). In order to determine the FSR, the following parameters will be measured in blood and muscle tissue: Plasma and muscle free phenylalanine concentration (expressed as *mol/L) Plasma enrichment of L-[ ring-2H5]phenylalanine (expressed as mole percent excess (MPE)) Muscle protein bound enrichment of L-[ ring-2H5]phenylalanine (expressed as MPE) L-[ ring-2H5]phenylalanine enrichment of the muscle free amino acid pool (expressed as MPE) | — |
Secondary
| Measure | Time frame |
|---|---|
| Secondary endpoints include whole body protein turnover, protein digestion and absorption kinetics and nitrate/nitrite analysis. The following parameters will be calculated: Exogenous phenylalanine rate of appearance and plasma availability of phenylalanine. Total rate of phenylalanine appearance and disappearance (= protein turnover) Endogenous phenylalanine rate of appearance (=protein breakdown) | — |
Countries
Netherlands
Outcome results
None listed