Skeletal muscle metabolism Skeletal muscle metabolism The metabolic process of the skeletal muscle
Conditions
Interventions
This is an intervention study, where each subject will undergo 12 days of
unilateral lower limb suspension. Randomly, the right or left leg of the
subject will be suspended by attachment of a sling
Sponsors
Universiteit Maastricht
Eligibility
Age
18 Years to 99 Years
Inclusion criteria
Inclusion criteria: Men between 18-35 years of age at time of enrolment Non smoking No recent bone fractures of the limbs No cardiovascular medication or other medication No (family) history of thrombosis No engagement in programmed exercise for more than two hours a week Stable dietary habits No contra-indication for MRI
Exclusion criteria
Exclusion criteria: Regular smokers Participation in other studies Female sex Recent bone fractures of the limbs Medication use Cardiovascular disease (Family) history of thrombosis Contraindications for MRI scans: • Electronic implants such as pacemaker or neurostimulator • Iron-containing foreign bodies in eyes or brain • Some hearing aids and artificial (heart) valves which are contraindicated for MRI • Claustrophobia Participants, who do not want to be informed about unexpected medical findings, or do not wish that their physician will be informed, cannot participate in the study
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| The main study parameters are the effects of lowered physical activity (resulting in decreased muscle mitochondrial oxidative capacity) on insulin sensitivity, lipid/glucose metabolism and intramyocellular lipid content. | — |
Secondary
| Measure | Time frame |
|---|---|
| Other study parameters: - Muscle strength test - Maximal whole body aerobic capacity (VO2max) - Body composition (DEXA) - Blood plasma levels of FFA, triglycerides and glucose - Leg circumference/Volume/ of quadriceps muscle - Leg skin temperature - Activity level (accelerometers) | — |
Countries
Netherlands
Outcome results
None listed