organprotection
Conditions
Interventions
The investigational intervention is a remote ischaemic conditioning stimulus.
Hereto, a pressure cuff will be placed on the upper arm and inflated to 200
mmHg for five minutes. Then, pressure will b
one baseline sample and
two samples after RIPC. Each blood withdrawal will be taken by a separate
venous puncture performed by a physician with extensive experience in this
field, such as an anesth
Sponsors
Academisch Medisch Centrum
Eligibility
Age
18 Years to 99 Years
Inclusion criteria
Inclusion criteria: Healthy, male volunteers aged 18-45 years.
Exclusion criteria
Exclusion criteria: Cardiovascular diseases, Alcohol or drug abuse, Fibrinolytic treatment in the previous 30 days, Usage of the anti-diabetic drug glibenclamide (this drug is known to block any conditioning effect). Doing any kind of sports on the night before the experimental day in the AMC
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| The primary endpoint of this study will be apoptosis, caspase 3/8/9 activity and LDH release measured at different time points in our in vitro models. | — |
Secondary
| Measure | Time frame |
|---|---|
| The secondary endpoints will be targets that will be analyzed from the Reperfusion Injury Salvage Kinase (RISK) pathway. | — |
Countries
Netherlands
Outcome results
None listed