Ovarian cancer
Conditions
Interventions
None listed
Sponsors
Vrije Universiteit Medisch Centrum
Eligibility
Age
18 Years to 99 Years
Inclusion criteria
Inclusion criteria: Material from 30 patients diagnosed with ovarian cancer and samples from 30 healthy volunteers will be collected. ;Inclusion criteria for the two groups;1. Patients with ovarian cancer: ;a. Diagnosed with epithelial ovarian cancer b. > 18 years c. Treatment in VUMC d. Understanding of the Dutch or English language e. Not using any medication f. No thrombotic events in history g. Written informed consent ;2. Healthy volunteers: ;a. > 18 years b. Understanding of the Dutch or English language c. Not using any medication d. No malignancies in past e. No thrombotic events in history f. Written informed consent
Exclusion criteria
Exclusion criteria: 1. Patients with ovarian cancer: ;a.
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| This project concerns the relevance of vesicles in ovarian cancer as potential biomarkers and can be subdivided in the following research sections: 1) Microparticles and exosomes will be isolated from urine, ascites and blood and identified on basis of their size, density and ability to bind cell specific antibodies. This will be measured by the use of a flow cytometer. Microvesicles presence and characteristics will be analysed and compared with those of healthy volunteers. 2) The presence of different proteins will be analysed within the isolated microvesicles and in the remaining supernatant. Different immunostaining techniques will be applied, such as western blots, flow cytometer and ELISpot. We will compare protein expression pattern from vesicles from patients with ovarian cancer and healthy volunteers to explore the sensitivity (and specificity) of the potential markers. | — |
Secondary
| Measure | Time frame |
|---|---|
| 3) Depending on these results, collected samples may be used to investigate genetic material which is captured in the microvesicles. Specific RNA sequences within isolated microparticles or exosomes will be analysed by use of reverse transcription polymerase chain reactions (RT-PCR). Other methods such as micro-arrays may be used to further investigate microvesicle*s RNA and DNA. | — |
Countries
Netherlands
Outcome results
None listed