Adipokines gene expression in visceral adipose tissue in patients with esophageal adenocarcinoma

Adipokines gene expression in visceral adipose tissue in patients with esophageal adenocarcinoma - Adipokines gene expression in esophageal adenocarcinoma

Status

Recruiting

Phases

Unknown

Study type

Observational

Source

NL-OMON

Registry ID

NL-OMON36786

Enrollment

Registered

2010-09-02

Start date

2010-10-05

Completion date

Unknown

Last updated

2024-05-06

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

esophageal adenocarcinoma (cancer of the esophagus) and obesity

Interventions

None listed

Eligibility

Age

18 Years to 99 Years

Inclusion criteria

Inclusion criteria: Patients with newly diagnosed esophageal adenocarcinoma who are referred to surgery at the Erasmus Medical Center will be included in our study. Sex- and BMI-matched patients who are referred for surgery at the Erasmus Medical Center for other reasons than esophageal cancer/ Barrett*s disease/GERD will be included as controls.

Exclusion criteria

Exclusion criteria: - Patients with severe weight loss (>10% during the last 3 months before surgery) - Patients without information on height and weight at the time of surgery - Patients with a BMI

Design outcomes

Primary

Measure	Time frame
Collected samples will be immediately frozen in liquid nitrogen and stored at -80°C until total RNA preparation. Total RNA will be extracted from adipose tissue and expression levels of the genes of interest are quantified with real-time-quantitative-PCR (RTq-PCR). We will use at least 2 housekeeping genes as an endogenous reference to compensate for variations in input RNA amounts.	—

Secondary

Measure	Time frame
Dependent on the results of our study on gene expression we will possibly measure the size of adipocytes in those 2 groups later in this study. Previously validated methods include: direct microscopy and manual/automated measurement of diameters from digital images. Influence of other patient characteristics on adipokines production/development of cancer: Patient information on prior weight loss, tumor stage and chronic diseases will be collected from the hospital information system and preoperative status. Other study parameters: Patient information on smoking, alcohol use, use of PPI and LES-relaxing and other drugs, history of reflux, DM, preoperative plasma-glucose-values as well as levels of HbA1c, cholesterol, triglycerides, CRP etc. will be collected from the patients* charts/ Elpado hospital information system if available.	—

Measure

Time frame

Dependent on the results of our study on gene expression we will possibly measure the size of adipocytes in those 2 groups later in this study. Previously validated methods include: direct microscopy and manual/automated measurement of diameters from digital images. Influence of other patient characteristics on adipokines production/development of cancer: Patient information on prior weight loss, tumor stage and chronic diseases will be collected from the hospital information system and preoperative status. Other study parameters: Patient information on smoking, alcohol use, use of PPI and LES-relaxing and other drugs, history of reflux, DM, preoperative plasma-glucose-values as well as levels of HbA1c, cholesterol, triglycerides, CRP etc. will be collected from the patients* charts/ Elpado hospital information system if available.

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Countries

Netherlands

Outcome results