Crohn's disease Inflammatory Bowel Disease Ulcerative Colitis
Conditions
Interventions
None listed
Sponsors
Academisch Medisch Centrum
Eligibility
Age
18 Years to 99 Years
Inclusion criteria
Inclusion criteria: *18 year old male or female, able to give informed consent Established Crohn*s disease or ulcerative colitis (according to the Lennard-Jones criteria), or patients scheduled for diverting ostomy due to IBD. control patients: patients who undergo intestinal resection due to a gastrointestinal malignancy
Exclusion criteria
Exclusion criteria: Ischemia of the bowel Positive stool cultures or parasite tests for common enteric pathogens (with exeption of non pathogenic parasites such as Blastocystis hominis or Endolimax nana) Use of Antiobiotics in preceding 4 weeks use of probiotics in preceding 8 weeks
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| - Nature and transmural spatial distribution of the enteric microbiota in IBD patients. - Changes in mucosal microbiota of the sigmoid in IBD after diverting ostomy. - Phenotype and characterise the immune cells involved in IBD. | — |
Secondary
| Measure | Time frame |
|---|---|
| - Presence of microbial DNA in granuloma*s of CD patients. - Assessment of co-localisation and signalling between invading microbes, lamina propria macrophages dendritic cells and adaptive and innate immune cells. - Alterations of phagosome maturation and autophagy, as well as apoptosis of mucosal macrophages and dendritic cells in IBD. | — |
Countries
Netherlands
Outcome results
None listed