dysfunctie van abdominaal vetweefsel dysfunctional abdominal fat dysfunctional visceral adipose tissue
Conditions
Interventions
None listed
Sponsors
Universitair Medisch Centrum Utrecht
Eligibility
Age
18 Years to 99 Years
Inclusion criteria
Inclusion criteria: 1. Between ages of 18 and 65 years. 2. Healthy volunteers 3. Male or female
Exclusion criteria
Exclusion criteria: 1. Known cardiovascular disease 2. Known renal, liver or pulmonary disease 3. Use of medication (except for oral anticonceptives, PPI*s, inhalation medication or topical unguents) 4. Pregnancy or lactation 5. Severe claustrophobia 6. Waist circumference too large to fit MRI. (> 200cm) 7. Metallic devices in the body
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| 1. Unit defining the outcome of the MR-spectroscopic measurements. Again, because MR-spectroscopy has not been performed on abdominal adipose tissue before, no predefined unit is available. With the spectroscopy measurements of abdominal adipose tissue, we want to be able to distinguish different lipid components at their known place in the spectrum. These peaks should include: - the water peak at 4.65 ppm - the triglyceride peak at 0.9 and 1.3 ppm - the total unsaturated fatty acids peak at 5.4 ppm - the poly unsaturated fatty acids peak at 2.8 ppm These peaks should be easily determinable and should not overlap each other , making calculation of AUC*s possible. If these conditions are met, data will be regarded as adequate. If other peaks are present in the spectra of the abdominal adipose tissue, these will be analyzed in the same manner. With calculated AUC*s of each of these components ratio*s of the lipid components relative to water can be calculated. These ratio*s in abdominal adipose tissue can be compared to the same ratio*s obtained from spectroscopic measurements in other tissues, for example subcutaneous adipose tissue or hepatic tissue. When related to other tissue*s a comparison can be made regarding the amount of the different lipidcomponents. For this study, because we do not know what will be the most appropriate tissue to compare the spectroscopic measurements of abdominal adipose tissue to, we want to perform spectroscopy of liver, subcutaneous adipose tissue, skeletal muscle and spleen. Because we want to determine whether the ratio's differ when a person has a small or large amount of abdominal adipose tissue, half of the participantis will have a waistcircumference of >88cm (women) or >102cm (men). 2. Reproducibility of spectroscopic measurements Both interscan (i.e. intra-individual) and interobserver reproducibility will be defined using Bland-Altman plots and interclass correlation coefficients. | — |
Secondary
| Measure | Time frame |
|---|---|
| 1. To compare spectroscopic measurements of participants with and without (laboratory) measurements of metabolic syndrome. 2. To compare adipokine profiles of participants with spectroscopic measurements (only when technique seems feasible). | — |
Countries
Netherlands
Outcome results
None listed