ischemia/reperfusion injury Kidney transplantation
Conditions
Interventions
None listed
Sponsors
Medisch Universitair Ziekenhuis Maastricht
Eligibility
Age
18 Years to 99 Years
Inclusion criteria
Inclusion criteria: - Subjects must sign the informed consent document prior to performance of any study related procedure including the Screening procedure. - Received kidney from; 1) living donor (n=12), 2)including the donor (n=12), brain-dead donor (n=12) and from donors after cardiac death (n=12). - Males and females >= 18 years of age.
Exclusion criteria
Exclusion criteria: - Smoking and a current history of smoking in the past six months. - Donor age 60 years.
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Renal perfusion: 1. Renal artery flow measured by Laser-Doppler flow probe. 2. Microcirculatory perfusion measured by OPS imaging (parameters: volumetric blood flow, functional capillary density). | — |
Secondary
| Measure | Time frame |
|---|---|
| Kidney function: 1. Dialysis dependency after transplantation. 2. Area under the curve for serum creatinine concentration over time (14 days). 3. Fractional excretion of sodium (14 days). Inflammation: Serum cytokine concentrations (IFN-γ, TNF-a, IL-6, IL-10, IL-17, IL-23). Renal cellular injury: Urine NGAL and NAG concentrations. Anti-oxidants: vitamin C, vitamin E, GSH/GSSG ratio, total anti-oxidant capacity. Endothelial glycocalyx: 1. Thickness measured by OPS imaging 2. Renal arterio-venous differences in syndecan-1, heparin sulphate and hyaluronan concentrations. | — |
Countries
Netherlands
Outcome results
None listed