Geen aandoeningen
Conditions
Interventions
Participants will receive a diet with usual macronutrient composition during
one week (approximately 15 en% protein, 55 en% carbohydrates and 30 en% Fat).
After this run in period participants will
Sponsors
Wageningen Universiteit
Eligibility
Age
18 Years to 99 Years
Inclusion criteria
Inclusion criteria: Age 18-40 years BMI between 18.5 and 30 kg/m2 Healthy (As judged by the participant) For women: using oral contraceptives Written informed consent obtained
Exclusion criteria
Exclusion criteria: Unusual dietary patterns, including high alcohol intakes (>= 2 glasses/day) and a vegetarian diet Usage of an energy restricted diet during the last two months Weight loss or weight gain of 5 kg or more during the last two months Smoking Any chronic disease Usage of any prescribed medication except for oral contraceptives For women: pregnant or lactating For women: Unwilling to use oral contraceptives for consecutively for at least the total study duration Liver function parameters (ALAT, ASAT, and γ-GT) and renal function parameters (Serum creatinin) outside the normal range
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Blood and 24h urines that are collected during the last day of each period will be explored for potential biomarkers of meat protein, dairy protein or grain and legume protein. Urine and blood will at least be analysed for potential biomarkers postulated in other studies, amino acids, and possibly other potential biomarkers that may be identified in future studies. Furthermore, we aim to identify new biomarkers through metabolomics using Nuclear magnetic resonance spectroscopy (NMR spectroscopy) and proteomics using mass spectometry. | — |
Secondary
| Measure | Time frame |
|---|---|
| - Net acid excretion will be measured in urine and computed from the diets. - Serum creatinine, 24h urinary creatinine and urinary albumin will be measured to estimate intrarenal pressure (=measure of hyperfiltration) - Excretion of sodium will be measured to estimate sodium retention. - In blood, volume distribution of ingested bromide will be measured to estimate extracellular volume | — |
Countries
Netherlands
Outcome results
None listed