cardiovascular disease
Conditions
Interventions
None listed
Sponsors
Universitair Medisch Centrum Sint Radboud
Eligibility
Age
18 Years to 99 Years
Inclusion criteria
Inclusion criteria: -Healthy young volunteers : age 18-30 -Healthy sedentary older volunteers: age >55 years + sedentary (55 years + trained (>5 h exercise per week) -All subjects: written informed consent
Exclusion criteria
Exclusion criteria: -Smoking -History of any cardiovascular disease -Hypertension (in supine position: systole >140 mmHg, diastole >90 mmHg) -Diabetes Mellitus (fasting glucose >7.0 mmol/L or random glucose >11.0 mmol/L) -Hyperlipidaemia (fasting total cholesterol >6.5 mmol/L) -Chronic use of medication known to interfere with the cardiovascular system -Professional athletes
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Change in endothelial function (measured with flow mediated dilation) after ischaemia reperfusion injury (induced by 20 minutes ischemia) with and without precedence of ischaemic preconditioning (by 3 cycles of 5-minutes of ischaemia). | — |
Secondary
| Measure | Time frame |
|---|---|
| Activation of PBMCs by plasma as measured in an ex-vivo assay. | — |
Countries
Netherlands
Outcome results
None listed