vetweefseldisfunctie bij obesitas obesity
Conditions
Interventions
None listed
Sponsors
Universiteit Maastricht
Eligibility
Age
18 Years to 99 Years
Inclusion criteria
Inclusion criteria: - All subjects entering the CO-Eur programme (life style intervention at the obesity treatment centre CO-Eur in Heerlen). Co-Eur only includes subjects with age>18 years and BMI>30kg/m2 - No more than 3 kg weight change in the past 3 months - age>18 years
Exclusion criteria
Exclusion criteria: - Smoking * Diabetes * (Medicinal) use of β-blockers or β-agonists * Pregnant women - (medicinal) use of anticoagulantia or blood clotting problems
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| For the pre- and post-measurement a subcutaneous adipose tissue biopsy of about 1 gram will be taken. one part will be used to measure sensitivity to β2-AR stimulation of lipolysis with salbutamol in adipocytes isolated from the biopsy. | — |
Secondary
| Measure | Time frame |
|---|---|
| Another part of the adipose tissue biopsy will be used to measure markers of local adipose tissue hypoxia and oxidative stress with western blot and PCR techniques. In addition a 5 ml venous blood sample and a small urine sample will be collected at both occasions. Markers of systemic oxidative stress will be measured in plasma and urine samples. | — |
Countries
Netherlands
Outcome results
None listed