chronic Q-fever Coxiella burnetii infection
Conditions
Interventions
None listed
Sponsors
Universitair Medisch Centrum Sint Radboud
Eligibility
Age
18 Years to 99 Years
Inclusion criteria
Inclusion criteria: Chronic Q-fever patients: - Proven or probable chronic Q fever according to the recent consensus of the Dutch workgroup on Q-fever;Q-fever fatigue syndrome (QFS) patients: - Laboratory-proven acute Q fever in the 3 years before presentation and/or positive serology fitting a past infection with Coxiella burnetii - AND being severely fatigued, defined by scoring 35 or higher on the subscale fatigue severity of the CIS - AND being fatigued for at least 6 months;Patients with past Q-fever infection without chronic infection or QFS - A history of laboratory-proven acute Q-fever infection by either PCR on sera or serology fitting acute infection - No chronic sequelae at least after 12 months of follow up;Healthy serologic negative volunteers: - Negative Q-fever serology;Vascular patients with chronic Q-fever undergoing surgery for infected atherosclerotic aorta aneurysm (AAA) : - Proven chronic Q-fever infection with infection of the vascular wall seen on CT, PET-CT or ultrasonography - Necessity of resection;Vascular patients undergoing surgery, either with AAA or AOD - For patients with uninfected AAA: An aneurysm of the aorta abdominalis for which resection with prosthetic replacement is necessary - For patients with AOD: An obstructive defect in the aorta or iliacal artery for which resection with prosthetic replacement is necessary ;Seropositive individuals reporting no history of Q-fever - Serology fitting past infection - No reported history of acute Q-fever ;Seropositive individuals with a history of acute Q-fever - A history of proven acute infection by either PCR on sera or serology fitting acute infection - No chronic sequelae at least after 12 months of follow up;Seropositive individuals with QFS - Laboratory-proven acute Q fever in the 3 years before presentation and/or positive serology fitting a past infection with Coxiella burnetii - AND being severely fatigued, defined by scoring 35 or higher on the subscale fatigue severity of the CIS - AND being fatigued for at least 6 months;Seropositive indivuals with chronic Q-fever - Proven or probable chronic Q fever according to the recent consensus of the Dutch workgroup on Q-fever;Seronegative individuals (controls) living in the endemic area without a history of Q-fever - Negative Q-fever serology - Living in endemic area
Exclusion criteria
Exclusion criteria: Age
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| 1. Circulating cytokine profiles in serum of patients during different stages of the various clinical manifestations of Q-fever. 2. In-vitro recognition and signalling pathways, cytokine pattern, Th-cell differentiation and macrophage polarisation of peripheral blood immune cells and immune cells isolated from aneurismal aorta wall, after stimulation with C. burnetii components. 3. Immunohistochemical properties of immune cells in aorta specimens of (Coxiella-infected) AAA patients and mRNA analysis for genes encoding proteins involved in the recognition and signalling of C. burnetii. 4. Polymorphisms in innate and adaptive immune response genes that may be related to the susceptibility and/or the development of a clinical manifestation of Q-fever. | — |
Secondary
| Measure | Time frame |
|---|---|
| n.v.t. | — |
Countries
Netherlands
Outcome results
None listed