Alpha1-antitrypsin deficiency, respiratory infections and airway inflammation: the role of epithelial cells

Alpha1-antitrypsin deficiency, respiratory infections and airway inflammation: the role of epithelial cells - AATDepithelial

Status

Active, not recruiting

Phases

Unknown

Study type

Observational

Source

NL-OMON

Registry ID

NL-OMON35677

Enrollment

Registered

2010-06-03

Start date

2020-10-01

Completion date

Unknown

Last updated

2024-07-22

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

COPD emphysema

Interventions

None listed

Eligibility

Age

18 Years to 99 Years

Inclusion criteria

Inclusion criteria: -FEV1 50 to 60% of predicted value measured after inhaled bronchodilators -A history of bronchial infections shown by at least 3 positive sputum cultures -Stable clinical condition in the preceding 3 months

Exclusion criteria

Exclusion criteria: -Asthma or any other lung disease -Use of oral steroids in the preceding 4 weeks

Design outcomes

Primary

Measure	Time frame
Bronchial biopsies will be analyzed by immunohistochemistry and digital image analysis for inflammatory cells. To allow further molecular and cellular characterization of Z-AATD-specific features of cultured bronchial epithelial cells, we will optimize a novel system to obtain bronchial epithelial cell lines with extended growth properties based on the introduction of the catalytic subunit of telomerase (hTERT) in these cells. Recent studies show that these immortalized human bronchial epithelial cells show a remarked ability for epithelial differentiation in culture, which is in contrast to other methods of immortalization previously reported.	—

Measure

Time frame

Bronchial biopsies will be analyzed by immunohistochemistry and digital image analysis for inflammatory cells. To allow further molecular and cellular characterization of Z-AATD-specific features of cultured bronchial epithelial cells, we will optimize a novel system to obtain bronchial epithelial cell lines with extended growth properties based on the introduction of the catalytic subunit of telomerase (hTERT) in these cells. Recent studies show that these immortalized human bronchial epithelial cells show a remarked ability for epithelial differentiation in culture, which is in contrast to other methods of immortalization previously reported.

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Countries

Netherlands

Outcome results