T cell homeostasis after ATG induced lymphocytopenia in renal transplant patients; studied through in vivo 2H2O labeling

T cell homeostasis after ATG induced lymphocytopenia in renal transplant patients; studied through in vivo 2H2O labeling - T cell homeostasis during lymphocytopenia

Status

Active, not recruiting

Phases

Unknown

Study type

Observational

Source

NL-OMON

Registry ID

NL-OMON35541

Enrollment

Registered

2010-12-22

Start date

2011-10-12

Completion date

Unknown

Last updated

2024-05-06

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

low white blood cells lymphocytopenia

Interventions

None listed

Eligibility

Age

18 Years to 99 Years

Inclusion criteria

Inclusion criteria: Renal transplantation patients treated with standard maintenance immunosuppressive therapy who were treated with ATG because of acute humoral graft rejection and renal transplantation patients treated with standard maintenance immunosuppressive therapy alone. Healthy volunteers, without relevant medical history.

Exclusion criteria

Exclusion criteria: Patients with intercurrent medical problems

Design outcomes

Primary

Measure	Time frame
The amount of 2H2O incorporated into the DNA within the naïve CD4+ and CD8+ T cells will be measured. With special mathematical formulas the production rate and half-life of cells can be calculated. T cell homeostasis in the ATG treated patients, versus the standard immunosuppressive treated patients and versus the healthy volunteers will be analysed through comparing production rates and half-life of the naïve T cells. For that purpose we will use methods that have been described in the literature, and we will collaborate with these same investigators.	—

Measure

Time frame

The amount of 2H2O incorporated into the DNA within the naïve CD4+ and CD8+ T cells will be measured. With special mathematical formulas the production rate and half-life of cells can be calculated. T cell homeostasis in the ATG treated patients, versus the standard immunosuppressive treated patients and versus the healthy volunteers will be analysed through comparing production rates and half-life of the naïve T cells. For that purpose we will use methods that have been described in the literature, and we will collaborate with these same investigators.

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Secondary

Measure	Time frame
Production rates and half-life of the CD31+ CD4 naive T cells.	—

Countries

Netherlands

Outcome results