Obesitas type 2 diabetes
Conditions
Interventions
None listed
Sponsors
Universiteit Maastricht
Eligibility
Age
18 Years to 99 Years
Inclusion criteria
Inclusion criteria: - Lean men (n=10; normoglycemic BMI 20-25 kg/m2) - Obese men (n=10 insulin resistant and n=10 insulin sensitive based on HOMA; BMI 30-35 kg/m2) - Aged 45-65 years - Caucasian - Normal blood pressure (systolic blood pressure 100-140 mmHg, diastolic blood pressure 60-90 mmHg) - Weight stable in last 3 months (± 2kg).
Exclusion criteria
Exclusion criteria: - Smokers - People with intensive fitness training, eg. athletes (>= 3 per week >= 1 hour training) - Serious pulmonary, cardiovascular, hepatic or renal disease - History of cardiovascular disease - Bleeding disorders - Current use of medication interfering with study endpoints/hypotheses (e.g. beta-blockers) - Not to be able to understand the study information - Subjects on a special diet or vegetarian - Blood donation 2 months prior to the study and during the study - Participation in other studies
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| The main study parameter is the effect of selective knockdown on glycerol release in the medium (as marker of lipolysis), TAG and DAG lipase activity, and lipid content in human adipocyte and myocyte cell cultures. | — |
Secondary
| Measure | Time frame |
|---|---|
| As secondary endpoints, differences in localization and trafficking of lipolytic proteins will be investigated in adipocytes derived from obese insulin resistant versus insulin sensitive men. | — |
Countries
Netherlands
Outcome results
None listed