Type 2 Diabetes mellitus
Conditions
Interventions
The intervention is a single exercise bout that consists of one hour one-legged
cycling on a adapted recumbent cycle ergometer at a submaximal rate. The
non-exercising leg will serve as control for
Sponsors
Wageningen Universiteit
Eligibility
Age
18 Years to 99 Years
Inclusion criteria
Inclusion criteria: • Age 40-60 years • Male gender • BMI
Exclusion criteria
Exclusion criteria: • Exercising regularly (> 2 times a week, > 3 hour in total per week) • Unstable body weight (weight gain or loss > 3 kg in the past three months) • Following, or have recently followed a (weight-loss) diet • Donated or intended to donate blood 2 months before until two months after the study • Medical condition that can interfere with the study outcome (i.e. cardiovascular disease, pulmonary disease) • Systolic blood pressure >160 mmHg and/or diastolic blood pressure >100 mmHg • Use of medications known to interfere with gene expression in the muscles (i.e. statins, fenofibrate) • Use of antithrombotic therapy (marcoumar, sintromitis). • Diagnosed diabetes mellitus type 1 or 2. • Drugs or alcohol abuse ( > 3 glasses of alcoholic beverages a day). • (Chronic) injuries of the locomotor system that can interfere with the intervention • Participated in another study within the last six months
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Main study outcomes include upregulation of genes in skeletal muscle after exercise (with a focus on genes encoding myokines) and changes of blood plasma levels of selected proteins after exercise. The proteins are selected based on gene expression changes measured. | — |
Secondary
| Measure | Time frame |
|---|---|
| PBMC gene expression: gene expression changes in the blood will be assessed Routine plasma levels: plasma levels of glucose, insulin, fatty acids, cortisol, adrenalin and lactate will be determined. Heart rate: heart rate will be measured. | — |
Countries
Netherlands
Outcome results
None listed