Time course and nature of nutrient sensing during fasting in humans

Time course and nature of nutrient sensing during fasting in humans - Time course nutrient sensing during fasting

Status

Active, not recruiting

Phases

Unknown

Study type

Observational

Source

NL-OMON

Registry ID

NL-OMON34127

Enrollment

Registered

2010-10-15

Start date

2011-01-17

Completion date

Unknown

Last updated

2024-05-06

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

n.a.

Interventions

None listed

Eligibility

Age

18 Years to 99 Years

Inclusion criteria

Inclusion criteria: Healthy males Age 19-29 yrs BMI 19-25 kg/m2 Stable weight for the last 3 months Caucasian FPG 7.5 mmol/l Negative family history (first degree) of DM2

Exclusion criteria

Exclusion criteria: Use of medication known to affect glucose metabolism (for example prednisone) or lipid metabolism Significant (chronic) disease Smoking (current) Alcohol consumption of more than 14 units per week at present or in the past Difficult accessible veins for insertion of an intravenous catheter Recent blood donation (within the last 3 months) Recent participation in other research projects (within the last 3 months), participation in 2 or more projects in one year Rigorous exercise/sports 5 or more days a week

Design outcomes

Primary

Measure	Time frame
• Plasma parameters: cortisol, IGF-1, free thyroxine, triiodothyronine, testosterone, oestradiol, SHBG, prolactin, TSH, GH, LH, FSH, ACTH, insulin, glucose, ketones, adiponectin, leptin, ghrelin. • Biochemical measures in skeletal muscle (m. vastus lateralis biopsy): phosphorylation state, enzyme activity and gene expression of AMPK, mTOR, SIRT1/2, FOXO1/3 and various up- and downstream targets of these enzymes and transcription factors. • Fuel oxidation (indirect calorimetry)	—

Measure

Time frame

• Plasma parameters: cortisol, IGF-1, free thyroxine, triiodothyronine, testosterone, oestradiol, SHBG, prolactin, TSH, GH, LH, FSH, ACTH, insulin, glucose, ketones, adiponectin, leptin, ghrelin. • Biochemical measures in skeletal muscle (m. vastus lateralis biopsy): phosphorylation state, enzyme activity and gene expression of AMPK, mTOR, SIRT1/2, FOXO1/3 and various up- and downstream targets of these enzymes and transcription factors. • Fuel oxidation (indirect calorimetry)

—

Secondary

Measure	Time frame
n.a.	—

Countries

Netherlands

Outcome results