Adipose tissue depot-specific differences in adipocyte function and inflammatory mediators in patients with type 2 diabetes mellitus and cardiovascular disease

Adipose tissue depot-specific differences in adipocyte function and inflammatory mediators in patients with type 2 diabetes mellitus and cardiovascular disease - Adipose tissue characteristics in patients with T2DM and in CVD

Status

Recruiting

Phases

Unknown

Study type

Observational

Source

NL-OMON

Registry ID

NL-OMON33838

Enrollment

Registered

2009-08-07

Start date

2010-01-05

Completion date

Unknown

Last updated

2024-05-06

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

Adipose tissue chronic low-graded inflammation inflammation of the adipose tissue

Interventions

None listed

Eligibility

Age

18 Years to 99 Years

Inclusion criteria

Inclusion criteria: Control subjects (males and females): Age: 40-60 yrs, BMI: 25-30 kg/m2, plasma glucose

Exclusion criteria

Exclusion criteria: Control group: presence of a chronic or acute inflammation and/or autoimmune disorder, insulin resistance;Type 2 diabetes mellitus (T2DM) patients: presence of a chronic or acute inflammation and/or another autoimmune disorder ;Subjects suffering cardiovascular disease: presence of another chronic or acute inflammation and/or autoimmune disorder, insulin resistance

Design outcomes

Primary

Measure	Time frame
To determine adipocyte (dys)function in both visceral and subcutaneous adipose tissues in the three different study groups: adipocyte differentiation capacity, insulin sensitivity, and cell surface in the two different adipose tissue depots. To determine the state of inflammation in both visceral and subcutaneous adipose tissues in the three different study groups: measure adipose tissue macrophage and lymphocyte infiltration, characterize the macrophage subsets in the visceral and subcutaneous adipose tissue.	—

Measure

Time frame

To determine adipocyte (dys)function in both visceral and subcutaneous adipose tissues in the three different study groups: adipocyte differentiation capacity, insulin sensitivity, and cell surface in the two different adipose tissue depots. To determine the state of inflammation in both visceral and subcutaneous adipose tissues in the three different study groups: measure adipose tissue macrophage and lymphocyte infiltration, characterize the macrophage subsets in the visceral and subcutaneous adipose tissue.

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Secondary

Measure	Time frame
Anthropometric measurements: BMI and WHR; the circumference of the waist as a measure of degree of abdominal obesity. Plasma levels of: insulin, glucose, free fatty acids, lipids (total triglyceride and total cholesterol with the VLDL, LDL and HDL sub fractions), adipokines (adiponectin, leptin etc.) and cytokines (IL-1, IL-6, IL-8, MCP-1, TNF-a etc.). Activation state of thrombocytes as a surrogate marker for cardiovascular risk.	—

Measure

Time frame

Anthropometric measurements: BMI and WHR; the circumference of the waist as a measure of degree of abdominal obesity. Plasma levels of: insulin, glucose, free fatty acids, lipids (total triglyceride and total cholesterol with the VLDL, LDL and HDL sub fractions), adipokines (adiponectin, leptin etc.) and cytokines (IL-1, IL-6, IL-8, MCP-1, TNF-a etc.). Activation state of thrombocytes as a surrogate marker for cardiovascular risk.

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Countries

Netherlands

Outcome results