Determination of protein profiles in vitreous of eyes with endophthalmitis with Surface Enhanced Laser desorption/ ionization time-of-flight (SELDI-tof) technology.

Determination of protein profiles in vitreous of eyes with endophthalmitis with Surface Enhanced Laser desorption/ ionization time-of-flight (SELDI-tof) technology. - Detection of infection with SELDI-tof in case of endophthalmitis

Status

Active, not recruiting

Phases

Unknown

Study type

Observational

Source

NL-OMON

Registry ID

NL-OMON33140

Enrollment

Registered

2009-07-07

Start date

2009-11-13

Completion date

Unknown

Last updated

2024-05-06

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

Infection of vitreous inflammation of ocular fluid

Interventions

None listed

Eligibility

Age

18 Years to 99 Years

Inclusion criteria

Inclusion criteria: Patients with endophthalmitis: - -Age >= 18 years -initiation of endophthalmitis within 6 weeks after cataract surgery;Controll group: -wils - Age >= 18 years -patient that undergo a pars plana vitrectomy because of a macular hole or macular pucker.

Exclusion criteria

Exclusion criteria: Endophthalmitis group: -patients able to give informed consent -previous uveitis in the affected eye -previous pars plana vitrectomy in the affected eye.;Controll group: macylar hle/ macular pucker -retinopathy because of Diabetes Mellitus or previous uveitis in the affected eye -previous pars plana vitrectomy in the affected eye.;-

Design outcomes

Primary

Measure	Time frame
Three analyses will be performed with SELDI-tof in comparison to regular culture methods: 1. Comparison of protein composition of vitreous in comparison to serum obtained bij surgery for maucular hole or macular pucker: controll population 2. Comparison of protein composition of vitreous in comparison to serum of patients with endophthalmtis 3. Comparison of protein composition of vitreous in comparison to serum of patients with endophthalmitis versus the controll population.	—

Measure

Time frame

Three analyses will be performed with SELDI-tof in comparison to regular culture methods: 1. Comparison of protein composition of vitreous in comparison to serum obtained bij surgery for maucular hole or macular pucker: controll population 2. Comparison of protein composition of vitreous in comparison to serum of patients with endophthalmtis 3. Comparison of protein composition of vitreous in comparison to serum of patients with endophthalmitis versus the controll population.

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Secondary

Measure	Time frame
1. Evaluation of the correlation between specific bacterial causes of endophthalmitis indentified by culture and specific protein pattern obtained via SELDI-tof analyses. 2. Evaluation of the correlation of vitreous of endophthalmitis with a negative result with culture and the protein pattern obtained by SELDI-tof analyses. We will compare the protein patterns of culture negative results with peak pattern of bacteriae identified by culture.	—

Measure

Time frame

1. Evaluation of the correlation between specific bacterial causes of endophthalmitis indentified by culture and specific protein pattern obtained via SELDI-tof analyses. 2. Evaluation of the correlation of vitreous of endophthalmitis with a negative result with culture and the protein pattern obtained by SELDI-tof analyses. We will compare the protein patterns of culture negative results with peak pattern of bacteriae identified by culture.

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Countries

Netherlands

Outcome results