obese en gezonde vrijwilligers the molecular and biochemical mechanism of fatty acids in blood cells
Conditions
Interventions
Elke participant will consume 3 high-fat milkshakes differering in fatty acid
composition (saturated fat, monounsaturated fat and polyunsaturated fat)
Sponsors
Wageningen Universiteit
Eligibility
Age
18 Years to 99 Years
Inclusion criteria
Inclusion criteria: Inclusion criteria for all participants: - male gender -50-70 yrs For diabetic patients only: - BMI >30 kg/m2 - Well-controlled diabetes: fasting plasma glucose concentration must be 30 kg/m2 - normoglycemic according to WHO criteria (OGTT, fasting blood glucose
Exclusion criteria
Exclusion criteria: Exclusion criteria for all participants: All subjects: - Female gender - Age below 50 or above 70 years - Hemoglobin levels 3 kg in the past three months) - Medical condition that can interfere with the study outcome (i.e. cardiovascular disease, gastrointestinal disease, renal dysfunction) - Use of medications know to interfere with glucose homeostasis (i.e. corticosteroids) - abuse of drugs and/or alcohol - participation in another biomedical study within 1 month before the first screening visit;For obese, type 2 diabetic subjects only: - severe diabetes which requires application of insulin - diabetes-related complications For obese subjects and lean controls only: - hyperglycemic according to WHO criteria (OGTT, fasting blood glucose >6.0mM, after 2 hr>11mM) -systolic blood pressure >160 mmHg or diastolic blood pressure > 100 mmHg - Urinary glucose concentrations (>0.25 g/l)
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| PBMC gene expression profiles | — |
Secondary
| Measure | Time frame |
|---|---|
| PBMC inflammatory response capacity Endothelial function Tertiar: lipid profiles plasma glucose and insulin monocyte and lymfocyte gene expression profiles | — |
Countries
Netherlands
Outcome results
None listed