glucose intolerance Insulin resistance
Conditions
Interventions
Glucose fluxes will be measured during a hyperinsulinemic clamp using stable
isotopes. Biopsies of skeletal muscle and subcutaneous adipose tissue will be
performed during the clamp. Biopsies of the
Sponsors
Academisch Medisch Centrum
Eligibility
Age
18 Years to 99 Years
Inclusion criteria
Inclusion criteria: Lean subjects: BMI 20-25 kg/m2 (stable weight) Age between 20 and 60 years Obese subjects: - BMI > 30 kg/m2 for part one or > 35 kg/m2 for part two - Age between 20 and 60 years - HOMA-IR > 1.95 for part one
Exclusion criteria
Exclusion criteria: Lean subjects: any medical condition (except for gallstone formation) or use of medication family history of diabetes mellitus type 2 performance of vigorous exercise Obese subjects: primary lipid disorder or secondary lipid disorder treated with fibrates diabetes mellitus type 2 (DM2) treated with thiazolidinediones, DPP4-inhibitors or GLP1-analogues any medical condition except for glucose intolerance, DM2, hypertension and secondary dyslipidemia
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Expression of RBP4 in liver, skeletal muscle and adipose tissue Glucose production, glucose uptake and lipolysis RBP-4 concentration in plasma | — |
Secondary
| Measure | Time frame |
|---|---|
| Correlation tissue levels of RBP4 and phosphorylation of insulin signaling proteins | — |
Countries
Netherlands
Outcome results
None listed